Investigation of Chemical Composition and Biological Activities of -Isolation of Iridoids and Phenylethanoid Glycosides.

Despite several studies on the L. genus, the chemical composition of , an alpine endemic species, is still largely unknown. The purpose of this study was to therefore deeper describe it, particularly from the phytochemistry and bioactivity perspectives.

Development of an innovative maceration technique to optimize extraction and phase partition of natural products.

The active component extraction from plants is the first crucial step in natural product research. For non-targeted extraction with an objective to isolate and characterize as many compounds as possible, the most classical technique, and the simplest to implement, is the Soxhlet extraction; however, it does not allow retrieving all the compounds from the plant (when it does not additionally cause artifacts during long heating process). The second most used technique is the extraction by successive macerations using solvents of increasing polarity.

Determination of suspected fragrance allergens extended list by two-dimensional gas chromatography-mass spectrometry in ready-to-inject samples.

A new strategy was developed to elucidate and quantify 56 (69 analytes including isomers) suspected chemically defined fragrance allergens in perfumes that were recently targeted by the Scientific Committee on Consumer Safety (SCCS). Samples were analyzed with a two-dimensional gas chromatography-quadrupole mass spectrometry system (GC-GC-MS). Method performance was evaluated by the accuracy profile approach to determine uncertainties around the regulation limit of 10mg/kg.