Co-Infection by and Serovar Pomona in Urine Samples of Donkeys and Pigs in Sardinia, Italy.

Saprophytic leptospires are spirochetes enclosed within the non-pathogenic clade of the genus which in turn is subdivided into two subclades S1 and S2. To date, the microorganisms included in these subclades have been isolated from the environment in various parts of the world, and are believed to have no known animal reservoirs. After a case of serovar Pomona was notified to the owner of a farm in Sardinia, all of the farm animals (11 pigs and 3 donkeys) were examined for the presence of .

First Isolation and Molecular Typing of Pathogenic and Intermediate Species from Urine of Symptomatic Dogs.

Aim of this study was to evaluate, the presence and diversity of spp. in blood and urine samples collected from 175 owned-dogs from Sardinia, Italy. After determination of leptospiral infection by microscopic agglutination test (MAT), urine from MAT-positive dogs were examined by real-time polymerase chain reaction ( rt-PCR) and then isolated by culture.

New Insights on Infections in a Canine Population from North Sardinia, Italy: A Sero-Epidemiological Study.

Leptospirosis is a widespread zoonosis recognized as a re-emerging infectious disease in a wide variety of animal species, including humans and dogs. No data exist regarding the presence of species in the canine population of Sardinia Island. This study reports the first sero-survey for leptospirosis in kennel and owned dogs from six areas of the north of Sardinia.

Molecular Typing of Pathogenic Species Isolated from Wild Mammal Reservoirs in Sardinia.

Leptospirosis is a global zoonosis caused by pathogenic species of that infect a large spectrum of domestic and wild animals. This study is the first molecular identification, characterization, and phylogeny of strains with veterinary and zoonotic impact in Sardinian wild hosts. All samples collected were cultured and analyzed by multiplex real time polymerase chain reaction (qPCR).