Publications by authors named "Aurea Valadares Folgueras-Flatschart"
J Virol Methods
September 2005
Article Synopsis
- A multiplex-PCR was developed to identify and distinguish between bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) using specific primers targeting the glycoprotein C gene.
- The technique was validated using both adapted viral strains and clinical samples from infected animals, confirming results with additional tests like virus isolation and semi-nested PCR.
- The method proved accurate, showing clear size differences in the amplified products for BoHV-1 and BoHV-5, and the internal control worked effectively with no cross-reactions to other viruses.
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