The Development of Monoclonal Antibody Against Thyroid-Stimulating Hormone for Congenital Hypothyroidism Screening in Indonesia.

Congenital hypothyroidism (CH) is a major health issue that can lead to intellectual disability if not detected and treated earlier. The preliminary screening program for neonatal CH in Indonesia gave a provisional incidence of 1:2513. Newborn screening using a dried blood spot sample is the standard method for CH detection, but it has limitations.

The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements.

Objective: Proper management of orthodontic treatment in diabetic patients is essential due to the heightened risk of periodontal tissue breakdown associated with hyperglycemia. Cacao bean extracts (CBE) are known to reduce the inflammatory response and increase synthesis and angiogenesis in periodontitis. Therefore, this study aims to examine the effect of CBE on preventing periodontal tissue breakdown in diabetes with orthodontic force.

The impact of Apis dorsata forest honey administration on follicle-stimulating hormone and luteinizing hormone levels in rats () forced swim test as a chronic physical animal model.

Background: Chronic physical stress has many effects on the nervous system and can cause structural changes in different parts of the brain and hemomodulatory, including hormonal. Current pharmacotherapeutic treatments have limited efficacy and are associated with many deleterious side effects.

Aim: The aim of this research is to determine how forest honey administration affects follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels in rats who are subjected to forced swim tests as a model of chronic physical stress placed in a container filled with water from which it cannot escape.

DLBS3233 enhances nephrin and podocin expression also reduces oxidative stress marker and insulin receptor serine diabetic rats' podocytes.

Unlabelled: Numerous oxidative stresses are detected in patients with diabetic kidney disease, resulting in insulin resistance that damages the pancreas and kidney. Renal podocytes insensitive to insulin lead to decreased nephrin and podocin and increased insulin receptor serine. The authors did an experiment on diabetic rats to examine the effect of DLBS3233 on repairing insulin resistance.

Molecular prevalence and genetic diversity of in free-range chicken in Northeastern Libya.

Background: is one of the zoonotic protozoa parasites. It can prevalently infect humans and warm-blooded animals, causing human health problems and substantial economic losses to the livestock industry worldwide. Chicken is one of the potential sources of toxoplasmosis, but there is no report of the prevalence of toxoplasmosis and their genotypes in free-range chickens in Libya.

induction method in adult zebrafish () as animal infection models.

Background And Aim: Zebrafish are frequently used as model organisms in scientific research as their genes mirror those of humans. bacteria can infect humans and animals, mainly fish. This study aimed to identify the concentration and route of infection in adult zebrafish.

Saponin and Fatty Acid Profiling of the Sea Cucumber , α-Glucosidase Inhibitory Activity and the Identification of a Novel Triterpene Glycoside.

Saponin-rich sea cucumber extracts have shown antidiabetic effects in a few reports. Although the triterpene glycosides of sea cucumbers are commonly isolated from their Cuvierian tubules, these are absent in Jaeger. Therefore, this study intended to investigate the saponin profile in the body wall of , as well as to assess the α-glucosidase inhibitory activity of the extracts.

Methanol extract of Black soldier fly () prepupae against and aureus bacteria and .

Background: and bacteria are pathogens in humans and animals. The therapy disrupts the virulence structure of the bacteria, resulting in bacterial death. Currently, chemical drugs have resulted in many resistant bacteria, so it is necessary to find alternative natural materials that are not toxic and do not quickly induce resistance.

The Characteristics of Demineralized Dentin Material Sponge as Guided Bone Regeneration Based on the FTIR and SEM-EDX Tests.

Objective:  The objective of this study was to determine the characteristics of demineralized dentin material sponge (DDMS).

Material And Methods:  An observational study was conducted on DDMS and BPCM. Fourier transform infrared (FTIR) test was performed to determine the characterizations of the materials.

Comparative performance of ELISA and dot blot assay for TSH-receptor antibody detection in Graves' disease.

Background: Graves' disease (GD) is an autoimmune disease, and it accounts for major cases of hyperthyroidism. Antibody against thyroid-stimulating hormone receptor/TSHR (TRAb) is responsible for hyperthyroidism and is considered as a diagnostic marker for GD. Therefore, we developed a recombinant protein of human TSHR-169 (hTSHR-169), which was specifically recognized TRAb in the serum of GD patients and then compare the diagnostic performance between ELISA and dot blot of TRAb tests for their ability to diagnose GD.

The early detection of type 1 diabetes mellitus and latent autoimmune diabetes in adults (LADA) through rapid test reverse-flow immunochromatography for glutamic acid decarboxylase 65 kDa (GAD).

Background: Diabetes mellitus (DM) is a chronic and costly disease that has become a primary concern worldwide. Type 1 diabetes mellitus is categorized as an autoimmune disease, which results in islet cell apoptosis and insulin-dependent. GAD is known as a potential marker of impaired pancreatic β cell function that appears in the initial phase of type 1 DM and latent autoimmune diabetes in adults (LADA).

Productivity, absence of a bull and endoparasitic nematodiosis in beef cattle farms in an upland area of East Java, Indonesia.

Background And Aim: Cattle are an important economic asset for the rural community in East Java Province, Indonesia. The study aimed to provide updated data of cattle farm demography, productivity, as well as the role of the absence of a bull and nematodiosis in reduced productivity of beef cattle in an upland rural area of the province.

Materials And Methods: The study was conducted in Sukowono village, Bondowoso region.

Data on attitudes, religious perspectives, and practices towards COVID-19 among Indonesian residents: a quick online cross-sectional survey.

Although previously large-scale social restrictions were implemented by the Indonesian government, the total number of coronavirus cases is overcome China in the global ranking per July 18th, 2020, implying a higher infection rate among Indonesian residents. The surge of new coronavirus cases started since the loosening of large-scale social restrictions, thereby implicating that public gathering (including religious gathering) evidently increases transmission [1]. It has been reported that Indonesia's coronavirus disease-19 (COVID-19) mortality rate is the second-highest among Southeast Asian Nations, which may be associated with several health determinants, including biochemical factors and health comorbidity [2], [3], [4], [5], [6], [7].

10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies.

Background We investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells. Methods For the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.

Cloning and Protein Expression of Gene in ESX-5 System from .

() is the causative agent of tuberculosis in human. One of the major virulence factors is early secretory antigenic target of 6-kDa (ESAT-6), and EccB protein encoded by is one of its components. EccB protein is a transmembrane protein in ESX-5 system.

Immune response evaluation in mice after crude extract of sensitization.

Background And Aim: is a global challenge for a fish product which may lead to a decrease in economic value and consumers' preference. Skipjack () in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. is one of the species which potent to induce an allergic reaction.

Diabetes sepsis on Wistar rat strain () induced by streptozotocin and bacteria .

Background And Aim: Sepsis is characterized by loss of control of the inflammatory response, which can be triggered by various microorganisms and toxic secretions. The mortality rate increases due to impaired endothelial function caused dysfunctional organ systems. Diabetes is closely related to sepsis.

Germline Mutations and Polymorphisms of Androgen Receptor in Prostate Cancer Patients: Frequency and Results of in Silico Analysis.

Background: Germline and somatic polymorphisms and mutations of the Androgen Receptor (AR) gene are known to be associated with the incidence of prostate cancer (PCa) in different populations. In this study we assessed germline AR polymorphisms and mutations in PCa patients with prediction of pathogenicity of the identified mutations by in silico analysis. Methods: Diagnosis of PCa was based on histopathology of prostate tissue (Gleason Score criteria) and serum prostate-specific antigen (PSA) levels.

SINGLE NUCLEOTIDE POLYMORPHISM OF GENE OF COMPLEX ISOLATES FROM SUSPECTED PULMONARY TB PATIENTS IN SURABAYA INDONESIA.

Background: Complex (MTBC) is a group of Mycobacterium that causes tuberculosis (TB). TB is an infectious disease that remains a global health problem. Indonesia is one of the five countries in the world where TB is the most prevalent and became the country with tle second largest rate of TB in 2014 and 2015.

Mechanism of retinal pericyte migration through Angiopoietin/Tie-2 signaling pathway on diabetic rats.

Aim: To investigate the mechanism of pericyte migration through Angiopoietin-2 (Ang-2)/Tie-2 signaling pathway.

Methods: We divided the rats into 5 groups. Each diabetic rat model groups injected with Tie-2 inhibitor, ERK1/2 inhibitor, Akt/PKB inhibitor, and DMSO intravitreal.

10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: and studies.

Objectives: Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells.