Publications by authors named "Augustinas Zelvys"
Article Synopsis
- Electrochemotherapy (ECT) is a targeted drug delivery method using pulsed electric fields that can struggle with uneven electric field distribution in tumors, leading to reduced treatment effectiveness.
- Researchers explored using 13 nm gold nanoparticles (AuNPs) to enhance electric field strength, aiming to address these non-homogeneity issues in ECT.
- The study found that AuNPs, when combined with specific pulsed electric fields, improved drug permeabilization and electrotransfer, enabling effective electrochemotherapy at lower electric fields without toxicity, showing promise for cancer treatment.
Gene electrotransfer (GET) is non-viral gene delivery technique, also known as electroporation-mediated gene delivery or electrotransfection. GET is a method used to introduce foreign genetic material (such as DNA or RNA) into cells by applying external pulsed electric fields (PEFs) to create temporary pores in the cell membrane. This study was undertaken to examine the impact of buffer composition on the efficiency of GET in mammalian cells Also, we specifically compared the effectiveness of high-frequency nanosecond (ns) pulses with standard microsecond (µs) pulses.
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- Ultra-short electric field pulses (nanoseconds) can selectively target mitochondria without affecting the outer cell membrane, showing promise for cancer treatments and influencing cell death and immune responses.
- The study compares the effects of longer (100 µs) and shorter (100 ns) pulse durations on mitochondrial function, measuring parameters like membrane potential, ATP levels, and reactive oxygen species (ROS) generation.
- Results indicate that while both pulse types affect mitochondrial activity, 100 ns pulses generate less ROS and can still be effective for calcium electrochemotherapy, highlighting their potential for targeted cancer therapies.
Calcium electroporation (CaEP) is an innovative approach to treating cancer, involving the internalization of supraphysiological amounts of calcium through electroporation, which leads to cell death. CaEP enables the replacement of chemotherapeutics (e.g.
View Article and Find Full Text PDFBovine colostrum (COL), the first milk secreted by lactating cows postpartum, is a rich source of bioactive compounds that exert a significant role in the survival, growth, and immune development of neonatal calves. This study investigated the immunomodulatory effects of COL on cytokine production in vitro using a Caco-2/THP-1 macrophage co-culture model stimulated with Phorbol 12-myristate 13-acetate (PMA). COL pretreatment significantly reduced IL-6 (241.
View Article and Find Full Text PDFGene delivery by the pulsed electric field is a promising alternative technology for nonviral transfection; however, the application of short pulses (i.e., nanosecond) is extremely limited.
View Article and Find Full Text PDFBovine colostrum (BC) is the first milk produced by lactating cows after parturition. BC is rich in various amino acids, proteins, and fats essential for the nutrition of the neonate calves. Despite the evident beneficial effect of BC on calves, the effect of BC on blood biomarkers is poorly understood.
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- The study investigates the use of bleomycin-based high-frequency nanosecond electrochemotherapy (nsECT) for treating LLC1 tumors in mice, demonstrating its effectiveness in tumor elimination and enhancing survival rates.
- Results indicate that nsECT increases immune cell populations and antitumor antibody levels post-treatment, suggesting a significant immunomodulatory effect.
- The treatment promotes specific immune responses, like upregulating PD 1 on CD4 Tr1 cells and enhancing various T cell and macrophage populations, indicating a potential combination of nsECT and immunotherapy for cancer treatment.
Objective: this work focuses on bleomycin electrochemotherapy using new modality of high repetition frequency unipolar nanosecond pulses.
Methods: As a tumor model, Lewis lung carcinoma (LLC1) cell line in C57BL mice (n = 42) was used. Electrochemotherapy was performed with intertumoral injection of bleomycin (50 μL of 1500 IU solution) followed by nanosecond and microsecond range electrical pulse delivery via parallel plate electrodes.
Pulsed electric field (PEF) is frequently used for intertumoral drug delivery resulting in a well-known anticancer treatment-electrochemotherapy. However, electrochemotherapy is associated with microsecond range of electrical pulses, while nanosecond range electrochemotherapy is almost non-existent. In this work, we analyzed the feasibility of nanosecond range pulse bursts for successful doxorubicin-based electrochemotherapy in vivo.
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