Hydrogen Sulfide Delivery to Enhance Bone Tissue Engineering Cell Survival.

Though crucial for natural bone healing, local calcium ion (Ca) and phosphate ion (P) concentrations can exceed the cytotoxic limit leading to mitochondrial overload, oxidative stress, and cell death. For bone tissue engineering applications, HS can be employed as a cytoprotective molecule to enhance mesenchymal stem cell (MSC) tolerance to cytotoxic Ca/P concentrations. Varied concentrations of sodium hydrogen sulfide (NaSH), a fast-releasing HS donor, were applied to assess the influence of HS on MSC proliferation.