Transcriptome analysis reveals the tolerant mechanisms to cobalt and copper in barley.

Cobalt (Co) and copper (Cu) co-exist commonly in the contaminated soils and at excessive levels, they are toxic to plants. However, their joint effect and possible interaction have not been fully addressed. In this work, a hydroponic experiment was performed to investigate the combined effects of Co and Cu on two barley genotypes at transcriptional level by RNA-seq analysis.

High accumulation of phenolics and amino acids confers tolerance to the combined stress of cobalt and copper in barley (Hordeum vulagare).

Cobalt (Co) and copper (Cu) co-exist in the metal contaminated soils and cause the serious toxicity to crops, while their interactive effect on plant growth and development is still poorly understood. In this work, a hydroponic experiment was carried out to reveal the interactive effect of Co and Cu on photosynthesis and metabolite profiles of two barley genotypes differing in metal tolerance. The results showed that both single and combined treatments of Co and Cu caused a significant reduction in chlorophyll content and photosynthetic rate of the two barley (Hordeum vulgare) genotypes, with the effect being greater for the combined treatment and the sensitive genotype (Ea52) being more affected than the tolerant genotype (Yan66).