Bioorthogonal Stimulated Raman Scattering Imaging Uncovers Lipid Metabolic Dynamics in Brain During Aging.

Studies have shown that brain lipid metabolism is associated with biological aging and influenced by dietary and genetic manipulations; however, the underlying mechanisms are elusive. High-resolution imaging techniques propose a novel and potent approach to understanding lipid metabolic dynamics . Applying deuterium water (DO) probing with stimulated Raman scattering (DO-SRS) microscopy, we revealed that lipid metabolic activity in brain decreased with aging in a sex-dependent manner.

Direct Imaging of Lipid Metabolic Changes in Ovary During Aging Using DO-SRS Microscopy.

Emerging studies have shown that lipids and proteins play versatile roles in various aspects of aging. High-resolution optical imaging provides a powerful approach to study the metabolic dynamics of lipids and proteins during aging. Here, we integrated DO probing and stimulated Raman scattering (DO-SRS) microscopy to directly visualize metabolic changes in aging ovary.