Determination of Antimicrobial Resistance Patterns in Salmonella from Commercial Poultry as Influenced by Microbiological Culture and Antimicrobial Susceptibility Testing Methods.

Monitoring antimicrobial resistance of foodborne pathogens in poultry is critical for food safety. We aimed to compare antimicrobial resistance phenotypes in isolated from poultry samples as influenced by isolation and antimicrobial susceptibility testing methods. isolates were cultured from a convenience sample of commercial broiler ceca with and without selective broth enrichment, and resistance phenotypes were determined for 14 antimicrobials using the Sensititre platform and a qualitative broth breakpoint assay.

Eimeria species and genetic background influence the serum protein profile of broilers with coccidiosis.

Background: Coccidiosis is an intestinal disease caused by protozoal parasites of the genus Eimeria. Despite the advent of anti-coccidial drugs and vaccines, the disease continues to result in substantial annual economic losses to the poultry industry. There is still much unknown about the host response to infection and to date there are no reports of protein profiles in the blood of Eimeria-infected animals.

Multi-platform next-generation sequencing of the domestic turkey (Meleagris gallopavo): genome assembly and analysis.

A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided a cost-effective assembly of the genome sequence of the domestic turkey (Meleagris gallopavo). Heterozygosity of the sequenced source genome allowed discovery of more than 600,000 high quality single nucleotide variants. Despite this heterozygosity, the current genome assembly (∼1.

Molecular cloning and functional characterization of avian interleukin-19.

The present study describes the cloning and functional characterization of avian interleukin (IL)-19, a cytokine that, in mammals, alters the balance of Th1 and Th2 cells in favor of the Th2 phenotype. The full-length avian IL-19 gene, located on chromosome 26, was amplified from LPS-stimulated chicken monocytes, and cloned into both prokaryotic (pET28a) and eukaryotic (pcDNA3.1) expression vectors.

Evaluation of the Polymerase Chain Reaction for the Detection of Salmonella enteritidis in Experimentally Inoculated Eggs and Eggs from Experimentally Challenged Hens.

Current recommendations for identification of Salmonella enteritidis (SE)-contaminated eggs, as outlined by the USDA Task Force, require the combination of yolk and albumen from several eggs for room-temperature enrichment for 3 days prior to culture on solid medium. We have previously reported the development of a technique involving enzymatic digestion and chemical reduction of pools of egg albumen allowing for the concentration of low numbers of by centrifugation. This technique allowed for detection of Salmonella with sensitivity comparable to conventional culture.

Evaluation of a Centrifugation Method for the Detection of Salmonella enteritidis in Experimentally Contaminated Chicken Eggs.

Current recommendations for identification of Salmonella enteritidis ( SE ) contaminated eggs, as outlined by the USDA SE Task Force, require the combination of yolk and albumen from several eggs for room-temperature enrichment for 3 days prior to culture on solid medium. We describe the use of enzymatic digestion and chemical reduction to reduce viscosity and allow for concentration of Salmonella cells in egg albumen by centrifugation prior to enrichment and plating. In these studies, sanitized, intact, fresh shell eggs were inoculated with low numbers of viable SE .