Publications by authors named "Audrey Mossler"
The eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre-replicative complex (pre-RC) assembly. Currently it is unknown how MCM2-7 ring closure and ATP-hydrolysis are controlled.
View Article and Find Full Text PDFOrigin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7 onto replication origins in G1-phase forms the basis of replication fork establishment in S-phase. However, how ORC and MCM2-7 facilitate genome-wide DNA licensing is not fully understood. Mapping the molecular footprints of budding yeast ORC and MCM2-7 genome-wide, we discovered that MCM2-7 loading is associated with ORC release from origins and redistribution to non-origin sites.
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- Metazoan chromosomes are organized into regions called TADs, which help regulate gene activity, but the exact mechanisms connecting these domains to transcription remain unclear.
- Using Capture Hi-C techniques, researchers examined changes in chromatin structure during mouse thymocyte development, finding specific remodeling events associated with gene activation.
- The study showed that gene transcription can influence chromatin structure, revealing that transcriptional processes play a key role in shaping how chromosomes are organized.