Publications by authors named "Audrey M Gonzalez"
Article Synopsis
- Adoptive cell therapy (ACT) using tumor-infiltrating lymphocytes (TIL) shows clinical efficacy in metastatic melanoma, with a 42% overall response rate across 74 patients.
- Prior treatment with anti-CTLA4 impacts TIL ACT outcomes negatively, leading to shorter survival and reduced response rates, particularly in patients who were pre-treated with this checkpoint inhibitor.
- Baseline serum IL9 levels may predict response to TIL ACT, suggesting a potential strategy for sequencing immunotherapies in treating melanoma patients.
Utilizing T-cell Activation Signals 1, 2, and 3 for Tumor-infiltrating Lymphocytes (TIL) Expansion: The Advantage Over the Sole Use of Interleukin-2 in Cutaneous and Uveal Melanoma.
René J Tavera Marie-Andrée Forget Young Uk Kim Donastas Sakellariou-Thompson Caitlin A Creasy Audrey M Gonzalez
J Immunother
September 2019
Article Synopsis
- The study proposes a new approach to improve tumor-infiltrating lymphocyte (TIL) therapy by speeding up the expansion process through a combination of T-cell receptor activation and stimulation of specific markers (CD137/4-1BB and IL-2).
- This 3-signal method resulted in rapid and consistent growth of CD8 TIL from melanoma tumors, achieving successful expansion in all cultures tested in less than three weeks.
- The enhanced TILs showed the ability to recognize and target melanoma cells, presenting a significant advancement for the application of TIL therapy in difficult-to-treat cancers like uveal melanoma.
Following the clinical success achieved with the first generation of adoptive cell therapy (ACT) utilizing expanded tumor-infiltrating lymphocytes (TILs), the second and third generations of TIL ACT are evolving toward the use of genetically modified TIL. TIL therapy generally involves the transfer of a high number of TIL, ranging from 10 to 10 cells. One of the technical difficulties in genetically modifying TIL, using a retroviral vector, is the ability to achieve large expansion of transduced TIL, while keeping the technique suitable to a Good Manufacturing Practices (GMP) environment.
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