Publications by authors named "Audrey J Klingele"
Meeting the goal of providing point of care (POC) tests for molecular detection of pathogens in low resource settings places stringent demands on all aspects of the technology. OmniAmp DNA polymerase (Pol) is a thermostable viral enzyme that enables true POC use in clinics or in the field by overcoming important barriers to isothermal amplification. In this paper, we describe the multiple advantages of OmniAmp Pol as an isothermal amplification enzyme and provide examples of its use in loop-mediated isothermal amplification (LAMP) for pathogen detection.
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- Researchers created Escherichia coli strains with extreme resistance to ionizing radiation (IR) through directed evolution in the lab.
- They identified 69 genetic mutations in a highly adapted strain, finding that just three mutations in specific DNA metabolism genes were primarily responsible for the increased IR resistance.
- The study emphasizes the importance of pre-existing DNA repair functions as a key mechanism in developing resistance to IR, suggesting that genetic adaptations can significantly enhance survival in extreme conditions.
Article Synopsis
- Viral metagenomic libraries can provide new enzyme reagents, and research on DNA from a hot thermal pool led to the discovery of a thermostable DNA polymerase called 3173 Pol.
- This enzyme has strong reverse transcriptase activity and includes a proofreading function for high accuracy in PCR applications.
- When tested against existing RT-PCR methods, 3173 Pol showed better specificity and sensitivity, making it a valuable option for research and diagnostics.
We have generated extreme ionizing radiation resistance in a relatively sensitive bacterial species, Escherichia coli, by directed evolution. Four populations of Escherichia coli K-12 were derived independently from strain MG1655, with each specifically adapted to survive exposure to high doses of ionizing radiation. D(37) values for strains isolated from two of the populations approached that exhibited by Deinococcus radiodurans.
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