Publications by authors named "Audrey E Fischer"
Article Synopsis
- * A biophysical fitness model, which considers capsid folding stability and antibody binding affinity, is used to predict how norovirus evolves to escape antibodies, and this model has been validated through experimental methods.
- * The findings reveal that competition for survival (selection) and random mutations (drift) influence viral evolution in different ways; specifically, they align along antibody binding affinity but oppose each other along capsid stability, with the balance influenced by the size of the viral population.
High mutation rates and short replication times lead to rapid evolution in RNA viruses. New tools for high-throughput culture and analysis of viral phenotypes will enable more effective studies of viral evolutionary processes. A water-in-oil drop microfluidic system to study virus-cell interactions at the single event level on a massively parallel scale is described here.
View Article and Find Full Text PDFUnlabelled: New human norovirus strains emerge every 2 to 3 years, partly due to mutations in the viral capsid that allow escape from antibody neutralization and herd immunity. To understand how noroviruses evolve antibody resistance, we investigated the structural basis for the escape of murine norovirus (MNV) from antibody neutralization. To identify specific residues in the MNV-1 protruding (P) domain of the capsid that play a role in escape from the neutralizing monoclonal antibody (MAb) A6.
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