Volume electron microscopy.

Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged, collectively called volume electron microscopy (vEM). Developments in vEM have been dubbed a quiet revolution as the field evolved from established transmission and scanning electron microscopy techniques, so early publications largely focused on the bioscience applications rather than the underlying technological breakthroughs.

Energy Cost of Children's Structured and Unstructured Games.

Background: Limited data are available on energy cost of common children's games using measured oxygen consumption.

Methods: Children (10.6 ± 2.