Effects of isoquinoline alkaloids as an alternative to antibiotic on oxidative stress, inflammatory status, and cecal microbiome of broilers under high stocking density.

This study investigated the effect of isoquinoline alkaloids as an alternative to bacitracin on growth performance, oxidative stress, inflammatory status, and ceca microbiome of broilers raised under high stocking density (HSD). A total of 1,500 one-day-old male Ross 308 chicks were randomly assigned to five treatment groups, with 10 replicate pens per group and 30 birds per pen, for 37 days. The treatments included normal stocking density (NSD, 10 birds/m²), HSD (15 birds/m²), HSD with 50 ppm Bacitracin (BCT50), HSD with 80 ppm isoquinoline alkaloids (IQA80), and HSD with 100 ppm isoquinoline alkaloids (IQA100).