In vitro validation concept for lyophilized fecal microbiota products with a focus on bacterial viability.

Fecal microbiota transplantation (FMT) effectively treats recurrent Clostridioides difficile infection (rCDI), typically administered as a fresh or frozen stool suspension through colonoscopy, nasojejunal tube, or oral capsules. Lyophilized fecal microbiota (LFM) are an alternative to frozen FM products. We aimed to assess whether lyophilization affects bacterial viability and metabolite levels and to develop LFM capsules for clinical use in Germany.

MF59-based lipid nanocarriers for paclitaxel delivery: optimization and anticancer evaluation.

Breast cancer is the most common invasive cancer in women worldwide, necessitating innovative therapeutic strategies to enhance treatment efficacy and safety. This study focuses on the development and optimization of novel paclitaxel (PTX)-loaded nanostructured lipid carriers (NLCs) that incorporate components of MF59, an oil-in-water emulsion adjuvant approved for use in influenza vaccines and known for its safety in humans. The formulation of these NLCs is designed to overcome significant challenges in PTX delivery, particularly its poor solubility and the side effects associated with traditional formulations containing Cremophor EL.

A Trihybrid Approach for Enhancing Crude Oil Recovery Using Effervescent-Tablet-Based Nanofluids.

While nanofluids can contribute enormously to enhanced oil recovery (EOR) in the upstream sector, conventional nanofluids are produced using complex equipment and expertise, which is somewhat limiting. To address this issue, herein, the use of effervescent-tablet-based nanofluids for EOR is reported. Tablets are formed by mixing and consolidating multi-walled carbon nanotubes, surfactants, and effervescent agents.

Management of a pediatric patient with dental anomalies and its effect on psychosocial status: a case report.

Managing multiple dental anomalies in the anterior region of the dental arch presents unique and complex challenges. This case report describes using a multidisciplinary approach to manage a child with not only a fused tooth with a supernumerary but also the presence of other supernumerary teeth in the same place. In this case, deviation from the standard management of a fused permanent anterior tooth, an anchor for aesthetics, by extracting it because of crown root complexity and allowing a supernumerary to erupt into its place made the difference in the successful outcome.

Improving full-arch implant scanning accuracy with a Meccano-like device for scan bodies splinting: A dental technique.

A long-term successful implant-supported prosthesis requires the creation of a passively fitting framework, based on an accurate intraoral digital implant scan. According to dental literature, splinted implant scan bodies (ISBs) provide higher intraoral scanning accuracy than non-splinted techniques. This technique presents a structured approach for splinting multi-unit abutment scan bodies (MUASBs) using customized manufactured devices to facilitate the recording of definitive intraoral implant scans.

Rare histopathological diagnosis of malakoplakia and Rosai‑Dorfman disease in the same uterus mimicking malignancy: A case report.

In the present case, a 66-year-old woman presented to the Specialty Hospital (Amman, Jordan) with recurrent post-menopausal bleeding. A pelvic ultrasound scan showed an abnormal endometrial thickness of 8 mm and no adnexal masses. An endometrial biopsy revealed abundant foamy histiocyte infiltration features suggestive of xanthogranulomatous endometritis.

Level of Awareness, Screening Practices, and Self-Detection Among Breast Cancer Patients.

Objective: Having good knowledge and performing regular pre-tests under physician supervision play a crucial role in the early detection of breast cancer. The aim of this study was to investigate the level of awareness, frequency of performing routine screening, types of screening methods prior to detection, and who detected the case, among women diagnosed with breast cancer.

Materials And Methods: A cross-sectional study that used a designed questionnaire applied to investigate demographic data and four other aspects: level of awareness, screening practices, type of screening methods used, and who detected the case for the first time.

High early strength concrete incorporating waste derived nanomaterials for sustainable construction.

This paper contributes to the expanding knowledge base on nanomaterial-enhanced cementitious composites, offering valuable insights for developing high-performance, sustainable concrete solutions. The study assessed the effects of three different types of nanomaterials-nano clay (NC), nano silica (NS), and nano cellulose (NCel)-on the compressive strength of high-early-strength concrete (HESC) through both experimental studies and a 2 factorial design. Incorporating nanomaterials into the HESC matrix led to a decrease in workability, with NCel demonstrating the least impact on this property across all studied replacement percentages.

Asparagine availability controls germinal center B cell homeostasis.

The rapid proliferation of germinal center (GC) B cells requires metabolic reprogramming to meet energy demands, yet these metabolic processes are poorly understood. By integrating metabolomic and transcriptomic profiling of GC B cells, we identified that asparagine (Asn) metabolism was highly up-regulated and essential for B cell function. Asparagine synthetase (ASNS) was up-regulated after B cell activation through the integrated stress response sensor GCN2.

Exploring the Potential of Electronic Patient-Generated Health Data for Evaluating Treatment Response to Intramuscular Steroids in Rheumatoid Arthritis: Case Series.

Background: Mobile health devices are increasingly available, presenting exciting opportunities to remotely collect high-frequency, electronic patient-generated health data (ePGHD). This novel data type may provide detailed insights into disease activity outside usual clinical settings. Assessing treatment responses, which can be hampered by the infrequency of appointments and recall bias, is a promising, novel application of ePGHD.

Combining machine learning, molecular dynamics, and free energy analysis for (5HT)-2A receptor modulator classification.

The 5-Hydroxytryptamine (5HT)-2A receptor, a key target in psychoactive drug development, presents significant challenges in the design of selective compounds. Here, we describe the construction, evaluation and validation of two machine learning (ML) models for the classification of bioactivity mechanisms against the (5HT)-2A receptor. Employing neural networks and XGBoost models, we achieved an overall accuracy of around 87 %, which was further enhanced through molecular modelling (MM) (e.

An impedimetric approach for determination of ammonium using silver/poly-1-aminoanthraquinone/carbon paste electrode.

Here, we present the electrochemical determination of ammonium in water samples, emphasizing the importance of accurate and precise assessment of its concentration. The modified electrode used in this study was fabricated through the anodic polymerization of 1-aminoanthraquinone (1-AAQ) and deposition of silver particles into a carbon paste electrode. The fabrication process involved cyclic voltammetry in a 0.

The study of single nucleotide polymorphism in a promoter region of ACE-2 receptor gene in the samples of Iraqi population with COVID-19.

Background: Angiotensin-converting enzyme 2 (ACE2) is an essential receptor on the host cell's cell membrane. It's interesting to note that the entry point receptor ACE2 protein and the severe acute respiratory syndrome (SARS) coronavirus are correlated. This study aimed to determine the influence of the ACE gene genotype and explore the effects of genetic variation in the promotor region of the ACE-2 gene receptor in SARS COV-2 patients.

Antimycotic prophylaxis with multispecies probiotics against oral candidiasis in new complete denture wearers: A randomized clinical trial.

Statement Of Problem: Changes in the oral microbiota of new complete denture wearers are the main cause of oral candidiasis. The drawbacks associated with traditional antimycotic therapies, especially drug resistance, have led to the search for potent therapeutic and prophylaxis agents with less harmful effects, including probiotics. However, investigation of the prophylaxis and preventive effects of probiotics on new complete denture wearers are lacking.

Unusual location of gastrointestinal stromal tumor (GIST): A case report and literature review of greater omentum location.

Introduction: Gastrointestinal stromal tumors (GISTs) represent a unique subset of neoplasms within the digestive tract. They can manifest in various organs throughout the digestive tract, ranging from the oral cavity to the anus, with a predilection for the stomach and small intestine. A distinct subtype of GISTs, known as Extra-gastrointestinal stromal tumors (EGISTs), originate outside the typical GIST organs such as the mesentery, retroperitoneum, and occasionally the omentum.

Caring for People Living With Dementia in Saudi Arabia: The Perspective of Nurses as Primary Caregivers.

Dementia is a progressive condition and an umbrella term used to describe a set of symptoms that affects many older adults. Older adults living with dementia often experience social stigma, which can impact their quality of life. Most people with dementia need the assistance of a caregiver in order to enhance their health.

A quantitative and qualitative analysis of patient group narratives suggests common biopsychosocial red flags of undiagnosed rare disease.

Background: The 'diagnostic odyssey' is a common challenge faced by patients living with rare diseases and poses a significant burden for patients, their families and carers, and the healthcare system. The diagnosis of rare diseases in clinical settings is challenging, with patients typically experiencing a multitude of unnecessary tests and procedures. To improve diagnosis of rare disease, clinicians require evidence-based guidance on when their patient may be presenting with a rare disease.

Synergistic optical sensing: Selective colorimetric analysis of copper in environmental and biological samples.

A groundbreaking optical sensing membrane has been engineered for the accurate assessment of copper ions. The pliable poly(vinyl chloride) membrane is formulated through the integration of sodium tetraphenylborate (Na-TPB), 4-(2-hydroxy-4-nitro azobenzene)-2-methyl-quinoline (HNAMQ), and tri-n-octyl phosphine oxide (TOPO), in conjunction with o-nitrophenyl octyl ether (o-NPOE). The sensor membrane undergoes a thorough investigation of its composition to optimize performance, revealing that HNAMQ serves a dual role as both an ionophore and a chromoionophore.

Robotic Cholecystectomy Remains Safe and Effective After Regular Staffing Hours.

Background Robotic-assisted surgery continues to grow in popularity. Access during evenings and weekends for non-elective operations can be restricted out of safety concerns. We sought to analyze and compare outcomes of patients undergoing robotic cholecystectomy, a common urgent procedure for acute calculous cholecystitis, during regular hours versus evenings or weekends.

A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution.

Frozen shoulder is a spontaneously self-resolving chronic inflammatory fibrotic human disease, which distinguishes the condition from most fibrotic diseases that are progressive and irreversible. Using single-cell analysis, we identify pro-inflammatory MERTKCD48 macrophages and MERTK + LYVE1 + MRC1+ macrophages enriched for negative regulators of inflammation which co-exist in frozen shoulder capsule tissues. Micro-cultures of patient-derived cells identify integrin-mediated cell-matrix interactions between MERTK+ macrophages and pro-resolving DKK3+ and POSTN+ fibroblasts, suggesting that matrix remodelling plays a role in frozen shoulder resolution.

Adverse Impact of Systemic Lupus Erythematosus on Pregnancy Outcomes: A Saudi Arabia Retrospective Multi-Center Study.

Objective: The current study aimed to determine the pregnancy outcomes complications in patients with SLE and its association with clinical, laboratory variables, disease activity, and medication use in the Saudi population, as well as pregnancy effect on disease activity.

Methods: A multicenter study included pregnant female patients with Systemic Lupus Erythematosus (SLE) from three tertiary centers in Saudi Arabia. The demographics, clinical, and laboratory variables, SLE disease activity index (SLEDAI), medication before, during, and after pregnancy, planned pregnancy, pregnancy-related outcomes, and complications in comparison to age-matched healthy female controls were noted.

Retraction: Epiploic Appendagitis Clinically Masquerading as an Acute Diverticulitis.

[This retracts the article DOI: 10.7759/cureus.20188.