Publications by authors named "Atsushi M Ito"
Article Synopsis
- Measuring mechanical forces of cell-cell interactions is key for understanding how multicellular organisms develop, but applying existing methods to tissues with cavities was uncertain.
- The study tested a previous image-based method using simulated data and found expanding cavities introduced additional repulsive forces in cell interactions, which correctly simulated cavity structures.
- Finally, the method was successfully applied to mouse blastocysts and two-dimensional cell sheets, demonstrating its effectiveness in modeling tissue morphologies with cavities.
Article Synopsis
- Mechanical forces play a crucial role in shaping the diverse 3D forms of multicellular systems, but understanding the specific cellular mechanical parameters influencing tissue morphology has been challenging due to limitations in live measurements.
- A new framework was created to model the mechanical forces in cell-cell interactions by treating cells as particles and deriving effective forces based on statistical analysis of cell tracking data.
- The study revealed that these interaction forces can be represented as pairwise potential energy that varies with cell distances, and significant differences in this energy profile exist among different species and embryonic stages, correlating with variations in their morphological characteristics.
Efficient and reliable estimation of the mean force (MF), the derivatives of the free energy with respect to a set of collective variables (CVs), has been a challenging problem because free energy differences are often computed by integrating the MF. Among various methods for computing free energy differences, logarithmic mean-force dynamics (LogMFD) [ Morishita et al., Phys.
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