Characterization of aminoglycoside resistance genes in multidrug-resistant Klebsiella pneumoniae collected from tertiary hospitals during the COVID-19 pandemic.

Since the peak of the coronavirus disease 2019 (COVID-19) pandemic, concerns around multidrug-resistant (MDR) bacterial pathogens have increased. This study aimed to characterize aminoglycoside resistance genes in MDR Klebsiella pneumoniae (K. pneumoniae) collected during the COVID-19 pandemic.

The Influence of Different Plasma Cell Discharges on the Performance Quality of Surgical Gown Samples.

An experimental study was performed on a low-density plasma discharge using two different configurations of the plasma cell cathode, namely, the one mesh system electrodes (OMSE) and the one mesh and three system electrodes (OMTSE), to determine the electrical characteristics of the plasma such as current-voltage characteristics, breakdown voltage (V), Paschen curves, current density (J), cathode fall thickness (d), and electron density of the treated sample. The influence of the electrical characteristics of the plasma fluid in the cathode fall region for different cathode configuration cells (OMSE and OMTSE) on the performance quality of a surgical gown was studied to determine surface modification, treatment efficiency, exposure time, wettability property, and mechanical properties. Over a very short exposure time, the treatment efficiency for the surgical gown surface of plasma over the mesh cathode at a distance equivalent to the cathode fall distance d values of the OMTSE and for OMSE reached a maximum.

Increasing frequency of Aminoglycoside-Resistant Klebsiella pneumoniae during the era of pandemic COVID-19.

The emergence of multidrug resistance to aminoglycosides in isolates is a growing concern, especially during pandemic Coronavirus disease 2019 (COVID-19). The study identifies antibiotic resistance in isolated from tertiary hospitals during pandemic COVID-19. Among 220 clinical isolates, the total rate of was found to be 89 (40.

Inactivation of Using Atmospheric Pressure Plasma Jet with Dry and Wet Argon Discharges.

The acceleration of inactivating viable cells of Escherichia coli (), by using new direct and indirect innovative methods, is the targeted method of using an atmospheric pressure plasma jet (APPJ) operated by an AC high-voltage power source with variable frequency up to 60 kHz and voltage ranging from 2.5 to 25 kV. Discharges using dry argon (0% O) discharges and different wet argon discharges using admixtures with O/Ar ratios ranging from 0.

An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia.

To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc).

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals.

Background And Objectives: Methicillin-resistant S. aureus (MRSA) tends to be resistant to multiple antibiotics. Methicillin resistance is conferred by the acquisition of the mecA gene, which is carried by a mobile genetic element called the staphylococcal cassette chromosome mec (SCCmec).

Frequency and antibiotic susceptibility of gram-positive bacteria in Makkah hospitals.

Background And Objective: Gram-positive bacteria are important nosocomial pathogens. The objective of this study was to estimate the frequencies and resistance rates of gram-positive pathogens isolated from hospitals in Makkah, Saudi Arabia.

Design And Setting: Prospective study at three Makkah hospitals from May 2008 to April 2009.

Profile of bacterial pneumonia during Hajj.

Background & Objectives: The congregation of a large number of people during Hajj seasons from different parts of the world in overcrowded conditions within a confined area for a long period of time presents many public health challenges and health risks. One of the main health problems of the crowding is ease transmission of pneumonia by air droplets. This study was aimed to determine the most common causes of bacterial pneumonia during the 2005 Hajj season and to relate the findings with clinical conditions.

The pobA gene of Burkholderia cenocepacia encodes a group I Sfp-type phosphopantetheinyltransferase required for biosynthesis of the siderophores ornibactin and pyochelin.

The opportunistic pathogen Burkholderia cenocepacia produces the siderophores ornibactin and pyochelin under iron-restricted conditions. Biosynthesis of both siderophores requires the involvement of non-ribosomal peptide synthetases (NRPSs). Using a transposon containing the lacZ reporter gene, two B.

Frequency and antimicrobial susceptibility of gram-negative bacteria isolated from 2 hospitals in Makkah, Saudi Arabia.

Objective: To estimate the prevalence and antibiotic susceptibility of the gram-negative bacteria isolated from 2 hospitals in Makkah.

Methods: This study was undertaken in 2 main tertiary care hospitals namely; Al-Noor Specialist Hospital, and Hera Hospital in Makkah, Kingdom of Saudi Arabia from October 2005 to March 2006. A total of 1137 gram-negative bacteria were identified in non-duplicate clinical specimens obtained from 965 patients of various body sites infections.

Frequency and antimicrobial susceptibility patterns of bacterial pathogens isolated from septicemic patients in Makkah hospitals.

Objectives: To determine the frequency of septicemic cases in Makkah hospitals, the main pathogens causing septicemia, and to describe the prevalence of antibiotic resistance among septicemia clinical isolates.

Methods: We performed a prospective study of 1626 septicemic cases in the 4 main hospitals in Makkah City during April 2004 to March 2005. Blood culture, isolation of organism and susceptibility to antibiotics were assessed using a routine microbiological methods.

Methicillin resistance among Staphylococcus aureus isolates from Saudi hospitals.

Objective: To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) strains among clinical isolates collected from the 4 tertiary hospitals in Makkah, Saudi Arabia, and to test the antimicrobial susceptibility patterns of S. aureus isolates against 9 antimicrobial agents.

Materials And Methods: A total of 512 S.

Accuracy of current oxacillin sensitivity tests routinely used in hospitals in Western Saudi Arabia.

Objective: To determine the accuracy of the current oxacillin resistant Staphylococcus aureus (S. aureus) detection test used in Makkah hospitals in comparison with the National Committee for Clinical Laboratory Standards (NCCLS) method.

Methods: A total of 500 S.

The Burkholderia cepacia fur gene: co-localization with omlA and absence of regulation by iron.

The ferric uptake regulator (Fur) functions as a transcription repressor of many genes in bacteria in response to iron, but the presence of a functional equivalent of this protein has not been demonstrated in Burkholderia cepacia. A segment of the Burkholderia pseudomallei fur gene was amplified using degenerate primers and used to identify chromosomal restriction fragments containing the entire fur genes of B. cepacia and B.