Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment.

Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.

Distribution of ubiquitinated histone H2A during plant cell differentiation in maize root and dedifferentiation in callus culture.

Although histone ubiquitination is known to associate with various chromatin functions, the precise role and mechanism of this modification remains still unknown. In this study, we identified the ubiquitinated form of H2A and estimated its ratio to total H2A in each of the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them, in order to define possible alterations, either during plant cell differentiation or during their dedifferentiation.