A tissue-engineered biocomplex for periodontal reconstruction. A proof-of-principle randomized clinical study.

Aim: To assess the safety/efficacy of a tissue-engineered biocomplex in periodontal reconstruction.

Methods: Twenty-seven intrabony defects were block-randomized across three treatment groups: Group-A (N  = 9) received autologous clinical-grade alveolar bone marrow mesenchymal stem cells (a-BMMSCs), seeded into collagen scaffolds, enriched with autologous fibrin/platelet lysate (aFPL). In Group-B (N  = 10), the collagen scaffold/aFPL devoid of a-BMMSCs filled the osseous defect.

In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts.

Purpose: The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.

Materials And Methods: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination.