Role of functional polymorphisms of NRAMP1 gene for the development of Crohn's disease.

Background: Crohn's disease (CD) is characterized by chronic activation of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) gene exerts many pleiotropic effects on macrophage functions. Hence, NRAMP1 may be also involved in the resistance to intracellular pathogens, and this effector of the innate immunity might be involved in CD pathogenesis.

Single nucleotide polymorphisms of OCTN1, OCTN2, and DLG5 genes in Greek patients with Crohn's disease.

Aim: To validate novel single nucleotide polymorphisms (SNPs) in Greek patients with Crohn's disease (CD).

Methods: A total of 120 patients with CD, 85 patients with UC, and 100 unrelated healthy controls were genotyped. Genotyping was performed by allele-specific PCR or by PCR-RFLP analysis.

Association of NOD2/CARD15 variants with Crohn's disease in a Greek population.

Objective: Single nucleotide polymorphisms in the NOD2/CARD15 gene have recently been shown to be associated with Crohn's disease (CD), but whether this susceptibility extends to all ethnic groups and geographic areas remains unknown. The aim of the present study was to evaluate the NOD2/CARD15 mutations in Greek patients with CD.

Methods: Individuals were genotyped for three NOD2/CARD15 mutations: R702W, G908R and L1007fsinsC.