Nitrate removal from industrial wastewater using six newly isolated strains of aerobic heterotrophic denitrifiers in an attached growth.

Background: The objective of this study was to isolate specific heterotrophic aerobic denitrifying bacteria from a wastewater treatment plant and employ them in an attached growth system for wastewater denitrification.

Methods: To isolate and screen aerobic denitrifiers, Denitrifying Medium (DM) and Screen Medium (GN) were utilized. The Polymerase Chain Reaction (PCR) technique and 16S rDNA sequencing were used to identify the isolates.