An integrated methodology for mining promiscuous proteins: a case study of an integrative bioinformatics approach for hepatitis C virus non-structural 5A protein.

A methodology for elucidation of structural, functional, and mechanistic knowledge on promiscuous proteins is proposed that constitutes a workflow of integrated bioinformatics analysis. Sequence alignments with closely related homologues can reveal conserved regions which are functionally important. Scanning protein motif databases, along with secondary and surface accessibility predictions integrated with post-translational modification sites (PTMs) prediction reveal functional and protein-binding motifs.