Anti-leishmanial polyclonal antibodies to assess the performance characteristics of leishmanial antigen detection ELISA.

The polyclonal antibodies raised in rabbits against amastigote antigen extract were purified and fractionated, and IgG class antibodies and from the same antibodies, a peroxidase conjugate (labeled antibodies) reagent were prepared. The antibodies and the labeled antibodies were analyzed for efficacy of the homologous extracted antigens by capture ELISA. The titration curves of the anti-amastigote IgG antibody against extracted antigens showed that both free antibody and corresponding labeled antibody reacted with the original amastigote antigens.