Placental blood flow and pregnancy outcomes in women with abnormal placental localization and absence of placental "migration".

Aim: We investigated the arcuate artery blood flow in the region of the abnormally localized placenta in women who had undergone insertion of an obstetric pessary and were receiving micronized progesterone.

Materials And Methods: The study included 120 pregnant women with high perinatal risks and abnormal placental localization. The patients were randomized to receive the Arabin's pessary and vaginal micronized progesterone (Group A,  = 60) or vaginal micronized progesterone only (Group B,  = 60).

3-Amino-thieno[2,3-b]pyridines as microtubule-destabilising agents: Molecular modelling and biological evaluation in the sea urchin embryo and human cancer cells.

A series of 3-amino-thieno[2,3-b]pyridines was prepared and tested in a phenotypic sea urchin embryo assay to identify potent and specific molecules that affect tubulin dynamics. The most active compounds featured a tricyclic core ring system with a fused cycloheptyl or cyclohexyl substituent and unsubstituted or alkyl-substituted phenyl moiety tethered via a carboxamide. Low nano-molar potency was observed in the sea urchin embryos for the most active compounds (1-5) suggestive of a microtubule-destabilising effect.

Synthesis and anti-mitotic activity of 6,7-dihydro-4H-isothiazolo[4,5-b]pyridin-5-ones: In vivo and cell-based studies.

A series of 3,7-diaryl-6,7-dihydroisothiazolo [4,5-b]pyridin-5(4H)-ones 8 and 9 was synthesized by multicomponent condensation of 3-aryl-5-isothiazolecarboxylic acid esters 4a-f with aromatic (or thienyl) aldehydes 7 and Meldrum's acid in an acidic medium. The targeted compounds were evaluated for their antimitotic microtubule destabilizing activity using in vivo phenotypic sea urchin embryo model and in vitro human cancer cell-based assays. Selected dihydroisothiazolopyridinones altered sea urchin egg cleavage in 2-10 nM concentrations together with significant cytotoxicity against cancer cells including chemoresistant cell lines (IC in submicromolar - low nanomolar concentration range).

A facile synthesis and microtubule-destabilizing properties of 4-(1H-benzo[d]imidazol-2-yl)-furazan-3-amines.

A series of 4-(1H-benzo[d]imidazol-2-yl)-furazan-3-amines (BIFAs) were prepared in good yields (60-90% for each reaction step) via a novel procedure from aminofurazanyl hydroximoyl chlorides and o-diaminobenzenes. The synthetic sequence was run under mild reaction conditions, it was robust and did not require extensive purification of intermediates or final products. Furthermore, there was no need for protection of reactive moieties allowing for the parallel synthesis of diverse BIFA derivatives.

Application of plant allylpolyalkoxybenzenes in synthesis of antimitotic phenstatin analogues.

Phenstatin and its derivatives with the modified ring A have been synthesized, using plant allylpolyalkoxybenzenes as a starting material. The targeted molecules were evaluated in a phenotypic sea urchin embryo assay for antiproliferative activity. It was found that phenstatin ring A modifications yielded antimitotic compounds.

Bone demineralization mechanisms at level of free radicals and nanoscale subsystems of bone tissue.

Influence of simulated microgravity on characteristics of rat bones was investigated by electron paramagnetic resonance (EPR). For simulation of microgravity condition the hanging of animal by tail was used. The main measurements were performed for diaphysis of femoral bones.

Metabolism in tooth enamel and reliability of retrospective dosimetry.

Most of the radiation centers in tooth enamel and bones are formed by the reactions COOH + gamma-->CO2- + H+, and CO2 + e- -->CO2-. These centers can be easily changed by metabolism and have a low stability in living organisms. The efficiency of CO2- radical production by irradiation is substantially different for teeth and bones in living rat, dead rat, and teeth and bone removed from rat.

Anisotropy effects of EPR signals and mechanisms of mass transfer in tooth enamel and bones.

Peculiarities of the internal construction of tooth enamel and bones that cause anisotropy effects and mass transfer in these objects are described. It is shown that the composition of the mineral component of teeth and bones depends on a mechanical-electrical mechanism, which pumps ions into nanocrystals. Decrease in the efficiency of the mechanical-electrical mechanism results in demineralization of enamel and bones, which progresses most rapidly at a disease of the biomineral or under special conditions, such as in space flights.