CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation.

The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4 iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and UKAi002-A-1, respectively) were generated. CXCL4 iPS cells showed deletion of exon 1 and complete loss of CXCL4 protein.

[Role of staphylococcus aureus hemolytic toxin-alpha in pathogenesis of infectious endocarditis: studies in vitro].

Aim: To study effects of alpha-toxin (AT) Staphylococcus aureus on human platelets and endothelial cells.

Materials And Methods: Concentrations of intracellular calcium in human platelets and endothelial cells were estimated by fluorescence, phosphoinositide metabolism in the endothelial cells was studied using 3H-myoinositole.

Results: AT induced a dose-dependent increase of intracellular calcium in blood and vascular cells, stimulates dose-dependent formation of inositol phosphates in endothelial cells.

[The functional characteristics of the thrombocytes in patients with hypertension and "silent" myocardial ischemia].

Functional platelet characteristics were studied in hypertensive subjects having episodes of silent myocardial ischemia. A total of 36 patients with essential hypertension (EH) stage II (WHO criteria, 1979) underwent echocardiography and 24-h ECG monitoring. Platelet aggregation induced by adenosine diphosphate was assessed by laser aggregation analyzer.

[A method for determining individual nifedipine sensitivity in treating hypertension patients].

Nifedipine antihypertensive effect was evaluated in 20 patients with essential hypertension. It was found to depend on pretreatment values of ADP-induced platelet aggregation response. The latter was studied with a new method of laser measurements of the aggregate radius sizes.

Altered sympathetic tone in hypertensives with angina and lowered threshold for pacing ischemia.

To determine the role of the sympathetic nervous system in myocardial ischemia with essential hypertension, plasma norepinephrine, heart rate (HR), blood pressure (BP), and the HR.BP double product at the time of silent ischemia during pacing and exercise treadmill test (ETT) were compared with basal values in 20 patients with sustained essential hypertension and stable angina, 3 to 60 days (12.6 +/- 11.

[Silent myocardial ischemia in patients with essential hypertension].

Holter monitoring was performed in 61 patients with essential hypertension. Painless, silent ST segment depression was found in 34 patients. Exercise myocardial scintigraphy indicated the occurrence of transient perfusion defects without abnormal clearance (Group 1) and those with abnormal clearance (Group 2).

[High-frequency jet ventilation of the lungs].

A study of high-frequency jet ventilation (HFJV) has been conducted on the lung model IS-800. Considerable changes in the tidal volume (TV) have been observed during HFJV with frequency of respiration (f) 60 and 100 min-1, which ensures non-monotonous respiration. Constant fluctuations of positive end-expiratory pressure (PEEP), maximum tracheal inspiratory pressure (Pmax.

[The use of microcomputers for calculating the indices of the hemodynamics, pump and contractile function of the left heart].

A programme is presented for the calculation of parameters reflecting pump and contractile functions of the left ventricular myocardium on Soviet-made microcomputer B3-34. The programme makes it possible to intensify studies 2.5-3-fold, assess hemodynamics many times at different stages of the investigation, obtain results and promptly give practical recommendations, which is of utmost importance in intensive care and resuscitation.

[ADP-induced thrombocyte aggregation in hypertension patients with different degrees of left ventricular hypertrophy].

Adenosine diphosphate (ADP)-induced platelet aggregation was studied in 59 patients suffering from essential hypertension, stages I and II (WHO, 1979) with different degree of left ventricular hypertrophy (LVH) by a new optical method. Using a laser aggregation analyzer the mean aggregate radius was measured in relative units. The left ventricular myocardial mass was estimated by echocardiography.

[Functional activity of the hypophysis and gonads in women with hypertension].

An attempt is made to demonstrate a relationship between changes in arterial blood pressure and sex steroids, gonadotrophins and mineralocorticoids in 30- to 55-year-old females with essential hypertension, employed in textile industry in Cheboksary. Climacteric and menopausal patterns of sex steroid and gonadotrophin secretion are discussed. A definite relationship between essential hypertension, elevated estradiol and testosterone and lowered gonadotrophins is demonstrated.

[Calcium metabolism in arterial hypertension. II. Changes in thrombocyte sensitivity to verapamil].

Verapamil produced dose-dependent inhibition of ADP- and TAF-induced increase in thrombocyte calcium concentration in hypertensive patients. Sensitivity to verapamil tested by EC50-half-maximal inhibition of responses to hormones can be weakened in vitro by adrenalin and strengthened by papaverin. Thrombocytes of hypertensive patients were individually sensitive to combination of papaverin and verapamil.

The hypertensive heart: pathogenesis, variants, and prognostic value.

We examine the heart's involvement in arterial hypertension, reporting on several studies of hypertensive patients showing that left ventricular myocardial mass is a significant prognostic indicator of essential hypertension and that left ventricular hypertrophy (LVH) correlates with an induced increase in Ca2+ concentration in platelets. We also consider the LVH variant of asymmetric hypertrophy and the role of the hypertensive heart in coronary insufficiency, and we speculate on the significance of the degree of LVH and asymmetric hypertrophy as risk factors for predicting cardiac complications of essential hypertension.

[Calcium metabolism in arterial hypertension. I. Myocardial hypertrophy and hormone-dependent increase in Ca2+ levels in the thrombocytes of patients with arterial hypertension].

Clonidine-treated patients with essential hypertension demonstrated reliable positive correlations between echocardiographically determined signs of myocardial hypertrophy (thickness of left ventricular posterior wall and interventricular septum, left ventricular myocardial mass) and hormone-dependent increase in thrombocyte calcium concentration induced by ADP and TAF. Antihypertensive therapy was more effective in patients with relatively weak cellular effect of Ca-increasing hormone response having no signs of myocardial hypertrophy.

[Effect of plasmapheresis on plasma hormone levels in patients with hereditary hypercholesterolemia].

It is shown that plasmapheresis in the volume of 30-46 ml/kg using 2997 cellular separator (IBM) does not significantly reduce testosterone, estradiol, aldosterone, parathyroid hormone and calcitonin concentrations. The initially high level of insulin and C-peptide was reduced, angiotensin-I concentration lowered and somatotropic hormone concentration doubled. It is suggested that plasmapheresis stimulates lipolytic and anabolic processes in the organism.