Publications by authors named "Ata Shirizadeh"
Background: The purity of the extracted DNA is critical for successful molecular testing. This study aimed to compare the effect of various DNA extraction methods, extraction processes, and sources of consumables, such as microtubes, on PCR results.
Methods: DNA extraction from whole blood was performed using four different approaches utilizing four types of microtubes: chloroform-based, sodium perchlorate-based, heat-assisted salting out, and solid-phase extraction.
Article Synopsis
- This study explored how specific genetic factors related to HLA-II alleles affect the presence of insulin autoantibodies in children with type 1 diabetes (T1D), involving a sample of 111 T1D children and their families.
- It found that certain HLA alleles (like DRB1*03:01 and DQB1*02:01) were more common in T1D patients, while other alleles were less frequent, indicating a possible genetic predisposition.
- The research also suggested that similarities between proteins from gut bacteria and islet cell autoantigens could contribute to the development of T1D, hinting that gut health may be an important factor for those at genetic risk.
Article Synopsis
- The study explores the link between specific HLA-II risk alleles and the development of Hashimoto’s thyroiditis (HT), using a sample of 100 HT patients and 330 healthy controls to identify predispositions and protective factors.
- It identifies several HLA alleles, particularly HLA-DRB1*03:01, *04:02, *04:05, and *11:04, as risk factors for HT, while finding that carrying these alleles increases the risk of developing the disease by 4.5 times.
- The research also reveals a significant sequence homology between thyroid autoantigens and herpes virus proteins, suggesting a potential link between viral infections and the onset of autoimmunity in HT
Article Synopsis
- A study was conducted on Iranian families with Type 1 diabetes (T1D) to analyze the genetic transmission of specific HLA alleles and haplotypes from parents to children and calculate the associated genetic risk score.
- The research involved 240 T1D subjects, including 111 parent-child trios, and compared them with 330 healthy individuals, using high-resolution HLA typing for detailed genetic analysis.
- Key findings showed that certain haplotypes significantly increased the risk of developing T1D, with one haplotype raising the risk by 24.5 times, while other haplotypes were found to offer protective benefits against the disease.