Publications by authors named "Aswin Rafif Khairullah"

Background: The LD (lethal dose causing 50% mortality) of ethylene glycol (EG) and its associated toxicity in mice () were assessed by evaluating kidney function.

Aim: This study aimed to determine the acute toxicity of an oral lethal dose of 50% (LD) of EG, also degeneration, necrosis, and inflammatory cell invasion in kidney tubules of male rats () as an animal model.

Methods: There were 66 DDG (Deutschland Denken Yoken) mice in 11 groups of six in this investigation.

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Monkeypox is a zoonotic disease caused by the virus, a double-stranded DNA virus that belongs the virus family. It is known to infect both animals (especially monkeys and rodents) and humans and causes a rash similar to smallpox. Humans can become infected with monkeypox virus (MPXV) when they get in close contact with infected animals (zoonotic transmission) or other infected people (human-human transmission) through their body fluids such as mucus, saliva, or even skin sores.

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Background: The TEM, CTX-M, and SHV are the result of mutations in the extended-spectrum beta-lactamases (ESBL) enzyme, which can be seen genotypically. Wild bats have never received antibiotic therapy, and the presence of antimicrobial-resistance (AMR) in bats indicates the level of AMR in the environment, the circulation of ESBL between humans or animals in Indonesia and wild animals is still not widely known. Whole genome sequencing is used to determine the circulation of ESBL in detail.

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Background: Due to its deadly toxins and ability to produce enzymes, the commensal bacterium is found in animals, birds, and people. It may also be linked to certain medical disorders

Aim: This study was aimed at the prevalence and molecular detection of A and D genes from livestock-associated methicilin-resistant (LA-MRSA) in raw chicken meats sold in Abakaliki.

Methods: The butchers in the slaughterhouses provided 150 fresh raw chicken meat samples, which were then cultured and purified using mannitol salt agar.

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Myostatin (MSTN) is an essential gene that regulates muscle growth and development in livestock animals, influencing meat production and carcass quality. This review looks at MSTN genetic diversity in a variety of animals, including cattle, sheep, goats, and poultry, focusing on correlations between particular mutations and phenotypic parameters such as muscle mass and growth rate. Mutations in the MSTN gene have been linked to desirable features such as greater body weight and higher meat quality, making it an ideal candidate for genetic selection in breeding programs.

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Avian pathogenic (APEC) causes colibacillosis in poultry; this type of bacteria is an extraintestinal pathogen . Unlike other pathogen groups, the characteristics of APECs cannot be identified by a single group. Serotyping and biotyping are frequently performed for isolates found in colibacillosis infections.

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One of the worst zoonotic illnesses, avian influenza (AI), or commonly referred to as bird flu, is caused by viruses belonging to the genus Influenza viruses, which are members of the Orthomyxoviridae family. The harmful effects of AI illness can affect both human and animal health and cause financial losses. Globally, the AI virus lacks political purpose and is not limited by geographical limits.

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Background: The most susceptible group of people to spread methicillin-resistant (MRSA) among domestic cats is their owners' relatives.

Aim: Considering the aforementioned, research at the Surabaya City Animal Hospital is necessary to determine whether the A gene may be detected in cat nasal swabs.

Methods: Samples were taken using a sterile cotton swab, and the transport medium was buffered peptone water.

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Paratuberculosis is an infectious disease caused by subspecies (MAP). Typically, ruminant animals including cattle, buffalo, goats, and sheep are infected with MAP. Animals get infected with MAP in a number of ways, such as by eating or drinking contaminated food or water, or by nursing from an infected mother who may have contaminated teats or directly shed the organism in milk or colostrum.

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Bovine trichomoniasis is a reproductive illness that affects cattle causing pyometra, early to mid-pregnancy miscarriages, and lower birth rates. is a flagellated protozoan which first discovered in France in 1888 and composts three phases during its lifecycle including trophozoite, cyst, and pseudocyst. In addition, several factors contributed to the prevalence of trichomoniasis and fall into three categories are management, cow, and bull-related factors.

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Background: is a known cause of a zoonotic infectious illness called toxocariasis. Parathenic hosts are important as they can transmit larvae 2 (L) through direct transmission. Scanning electron microscope (SEM) techniques are needed to provide a three-dimensional image of each stage of larvae.

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Article Synopsis
  • Kitchen sponges in homes are significant contributors to foodborne diseases due to their ability to harbor and transmit bacteria.
  • A study in Abakaliki metropolis, Nigeria, analyzed 50 kitchen sponges and found that over half were contaminated with multidrug-resistant bacteria, including Salmonella, Klebsiella, and E. coli.
  • The results highlight a serious public health issue, emphasizing the need for proper cleaning and disinfection of kitchen sponges to prevent the spread of resistant infections.
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The porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) belonging to the Arteriviridae family is the cause of PRRS disease. After being discovered for the first time in the United States in 1987, this illness quickly expanded to Canada. The disease was initially discovered in late 1990 in Germany, from where it quickly spread throughout Europe.

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The livestock-associated methicillin-resistant (LA-MRSA) strains are prevalent in the poultry farming environment and are a common component of the bacterial microbiota on the skin and mucous membranes of healthy animals. The origin and spread of LA-MRSA are attributed to the use of antibiotics in animals, and close contact between people and different animal species increases the risk of animal exposure to humans. The epidemiology of LA-MRSA in poultry significantly changed when ST398 and ST9 were found in food-producing animals.

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Classical swine fever (CSF), sometimes referred to as hog cholera, is a highly contagious, virally based, systemic illness that affects both domestic and wild pigs. The virus known as classical swine fever virus (CSFV) is a member of the Flaviviridae family, specifically the genus Pestivirus. This disease is thought to be endemic in many Asian countries that produce pork as well as in several countries in Central and South America, the Caribbean, and elsewhere.

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Article Synopsis
  • - Q fever, a zoonotic illness caused by bacteria, was first identified in 1935 in Queensland, Australia, and is considered a significant national health concern in many countries due to its contagious nature.
  • - It primarily spreads through inhalation, with some transmission via milk products, and is particularly risky for individuals working in veterinary and livestock settings.
  • - While antibiotics are effective for the acute form, chronic cases can be challenging to treat, and vaccination can help reduce infections; hygiene measures are crucial to control outbreaks.
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  • - IBR (infectious bovine rhinotracheitis) is a respiratory disease in cattle caused by the bovine alphaherpesvirus type 1 (BoAHV-1), which has multiple subtypes and causes serious health issues, including reproductive failures like abortions.
  • - The virus can remain dormant in cattle and reactivate under stress or illness, spreading through direct contact or via the reproductive system, and it can also be transmitted through contaminated semen.
  • - Diagnosis involves lab tests like cell culture and PCR, while management includes isolating infected animals and vaccination to prevent further spread and reduce symptoms.
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Background: Cases of cruelty can occur in wild animal, livestock, and pet animal. Cruelty to cats choose to be the background of this research because many cases of cruelty to cats have not been reported although the cases are still high.

Aim: The aim of this research was to know how much dilution of cat blood can still be detected by Leucomalachite Green (LMG) and Takayama reagent.

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Extended-spectrum β-lactamases (ESBLs) are β-lactamase enzymes produced by Gram-negative bacterial pathogens that harbor the ESBL genes. In addition, most ESBL genes are plasmid-mediated and usually encode a broader spectrum of antimicrobial resistance, especially to penicillins, first-generation, second-generation, and third-generation cephalosporins, as well as monobactam, such as aztreonam. has become an opportunistic pathogen, especially in poultry, and has been implicated in zoonotic diseases that can be transmitted to humans, resulting in public health problems.

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Campylobacteriosis is a foodborne illness that is contracted by eating contaminated food, particularly animal products like meat from diseased animals or corpses tainted with harmful germs. The epidemiology of campylobacteriosis varies significantly between low-, middle-, and high-income countries. Campylobacter has a complicated and poorly known survival strategy for getting past host barriers and causing sickness in humans.

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Salmonellosis, caused by species, is one of the most common foodborne illnesses worldwide with an estimated 93.8 million cases and about 155,00 fatalities. In both industrialized and developing nations, Salmonellosis has been reported to be one of the most prevalent foodborne zoonoses and is linked with arrays of illness syndromes such as acute and chronic enteritis, and septicaemia.

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Background: The Trichuris eggs are collected from naturally infected sheep. Natural antihelmintics such as herbal medicines are needed as an alternative, such as natural compounds from endemic plants.

Aim: This present study aims to evaluate the ovicidal activity and cytotoxicity effects of ethanolic extract of (EECL) and (EECS) as a biological anthelmintic against the egg of .

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Article Synopsis
  • Bovine tuberculosis is a zoonotic disease caused by bacteria that can infect both animals and humans, primarily affecting cattle and present globally except in Antarctica.
  • The article reviews aspects such as causes, symptoms, diagnosis (primarily via the tuberculin test), transmission, and its significant economic impact on the livestock industry.
  • Effective treatment includes first-line drugs like isoniazid and rifampin, while developed countries use regular testing and culling of infected animals to control the spread of the disease.
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One zoonotic infectious animal disease is brucellosis. The bacteria that cause brucellosis belong to the genus . Numerous animal and human species are affected by brucellosis, with an estimated 500,000 human cases recorded annually worldwide.

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Article Synopsis
  • This study focuses on identifying the invasive bacterial strain 0157:H7 from horse feces on Sumbawa Island using advanced molecular techniques, particularly the qPCR method.
  • Fresh fecal samples were collected and analyzed, revealing that six isolates tested positive for the 0157:H7 gene, with varying Cq and Tm values indicating different levels of bacterial presence.
  • The researchers emphasize the need for improved validation and standardization of qPCR technology to ensure accurate testing and effective disease management strategies.
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