Publications by authors named "Aswathi Cheredath"
Background: The unique epigenetic architecture that sperm cells acquire during spermiogenesis by retaining <15% of either canonical or variant histone proteins in their genome is essential for normal embryogenesis. Whilst heterogeneous levels of retained histones are found in morphologically normal spermatozoa, their effect on reproductive outcomes is not fully understood.
Methods: Processed spermatozoa (n = 62) were tested for DNA integrity by sperm chromatin dispersion assay, and retained histones were extracted and subjected to dot-blot analysis.
Context: The clinical value of human sperm metabolites has not been established due to the technical complexity in detecting these metabolites when sperm numbers are low.
Aims: To detect endogenous intracellular metabolites in fresh and post-thaw human spermatozoa using 800MHz nuclear magnetic resonance (NMR) spectroscopy equipped with a 1.7-mm cryo-probe.
Article Synopsis
- ICSI is a special method used to help men who have trouble having babies, but now it's being used a lot even when men aren't the problem, which is a bit too much.
- This study looked at embryos created by both IVF and ICSI from couples who didn’t have male fertility issues to see if they grow differently.
- The researchers found that after 96 hours, the embryos didn't show big differences in their chemical makeup, meaning ICSI doesn't change how the embryos develop compared to IVF in these cases.
Article Synopsis
- This study explored the integration of metabolomic and embryologic data with machine learning (ML) to enhance the prediction of embryo implantation success in infertile couples.
- The research involved 56 couples undergoing single blastocyst transfer, analyzing spent culture medium (SCM) to measure metabolite levels using NMR spectroscopy.
- Findings showed that lower levels of certain metabolites like pyruvate and threonine were linked to successful implantations, and a custom ML model achieved 100% accuracy in predicting embryo implantation potential, suggesting practical benefits for patient outcomes.
Background: The extended embryo culture using single-step medium gained popularity in clinical in vitro fertilisation (IVF). However, there are concerns about the degradation of unstable medium components and their negative effects on the developing embryos. Further, dry-incubation can increase osmolality, which can in-turn enhance the concentration of constituents of the media and their stability.
View Article and Find Full Text PDFBackground: The superficial sensory nerves are at great risk for injury from lacerations and operative incisions. Precise knowledge regarding the course and distribution of sensory nerves over the dorsum of foot is important to avoid iatrogenic damage by surgeons who are planning the surgical intervention around the distal leg, ankle and foot. The objective of the study is to report the variation in the branching pattern and distribution of the cutaneous nerves over the dorsum of foot.
View Article and Find Full Text PDFCryopreservation of immature-testicular-tissue (ITT) prior to gonadotoxic treatment, while experimental, is the only recommended option for fertility preservation in prepubertal boys. The handling and manipulation of ITT before cryopreservation could influence the functionality of cells during fertility restoration, which this study explored by evaluating cellular niche and quality of mouse ITT subjected to various temperatures and time durations in vitro. ITT from 6-day-old mice were handled at ultraprofound-hypothermic, profound-hypothermic, and mild-warm-ischemic temperatures for varying time periods prior to 14-day organotypic culture.
View Article and Find Full Text PDFInfertility affects approximately 15-20% of individuals of reproductive age worldwide. Over the last 40 years, assisted reproductive technology (ART) has helped millions of childless couples. However, ART is limited by a low success rate and risk of multiple gestations.
View Article and Find Full Text PDFBackground: Due to improved treatment, there is an increasing focus on the reproductive potential of survivors of childhood cancer. Cytotoxic chemotherapy accelerates the decline in the number of primordial follicles within the mammalian ovary at all ages, but effects on the developmental potential of remaining oocytes following prepubertal cancer treatment are unclear.
Objectives: To investigate whether cyclophosphamide (CY) exposure in the prepubertal period in female mice influences ovarian function and the functional competence of oocytes in adulthood.