Evaluation of Fitting Accuracy of Light- and Auto-Polymerizing Reline Materials Using Three-Dimensional Measurement Techniques.

Light-polymerizing reline materials offer improved chairside workability compared to conventional auto-polymerizing reline materials, addressing the partial denture (RPD) incompatibility caused by residual ridge resorption owing to long-term use. This study evaluates the fitting accuracy of relined materials by combining conventional fitting tests with three-dimensional (3D) measurements for detailed analysis. Light-polymerizing reline material (HikariLiner, Tokuyama, Tokyo, Japan, LP) and auto-polymerizing material (Rebase III, Tokuyama, AP) were used.

Identification of N-linked oligosaccharide labeled with 1-pyrenesulfonyl chloride by quadrupole time-of-flight tandem mass spectrometry after separation by micro- and nanoflow liquid chromatography.

The development of a qualitative determination method for the N-linked oligosaccharides in glycoproteins was attempted by the combination of micro- or nanoflow LC with Q-TOF-MS/MS. The asparaginyl-oligosaccharides in glycoproteins, liberated from the treatment of Pronase E, were separated, purified and labeled with 1-pyrenesulfonyl chloride (PSC). The resulting derivatives were separated by the microflow LC system utilizing a 0.

Rapid analysis of N-linked oligosaccharides in glycoproteins (ovalbumin, ribonuclease B and fetuin) by reversed-phase ultra-performance liquid chromatography with fluorescence detection and electrospray ionization time-of-flight mass spectrometry.

Rapid, selective and sensitive determination of N-linked oligosaccharides in glycoproteins (ovalbumin, ribonuclease B and fetuin) was performed by ultra-performance liquid chromatography (UPLC) with fluorescence (FL) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS). The asparaginyl-oligosaccharide moiety was first liberated from each glycoprotein by pronase E (a proteolitic enzyme). The oligosaccharide fractions separated by gel-permeation chromatography were labeled with 1-pyrenesulfonyl chloride (PSC, a fluorescence reagent), separated by UPLC in a short run time, and then detected by FL and TOF-MS.