Publications by authors named "Assis Rubens Montenegro"

The aim of the present study was to evaluate semen cryopreservation with ACP-Lact® diluent, which consists of coconut water powder (ACP) added to goat milk powder. After thawing, the samples were evaluated for sperm kinetics, membrane evaluation and insemination. For cryopreservation, a pool was made with the ejaculate of six goats, diluted in four equal aliquots for the respective treatments: T1 (ACP-Lact®); T2 (ACP-Lact® 50%); T3 (ACP + 2.

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Article Synopsis
  • The study investigated four non-permeable cryoprotectants (egg yolk, soy lecithin, sucrose, and lactose) for their effects on sperm cryopreservation.
  • The inclusion of egg yolk significantly improved sperm membrane integrity and motility compared to the control group.
  • The findings suggest that a combination of 5% egg yolk with glucose and dimethyl sulfoxide is the most effective approach for preserving sperm quality in this species.
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Here, we aimed to discriminate between the spectral profiles of spent culture media after oocyte maturation (IVM) and culture (IVC) from goats of different ages subjected to repeated hormonal treatments. The profiles were discriminated using near infrared (NIR) spectroscopy combined with multivariate methods. A total of 19 goats (young = 10; old = 9) were subjected to serial hormonal stimulation (HS) with gonadotropins.

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Mesenchymal stem cells (MSCs) from the umbilical cord (UC) have aroused considerable interest. However, little is known about the maternal effect on these cells. The aim of this study was to verify the impact of the nutritional status of donor goats on the growth and differentiation of MSCs from the UC.

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Article Synopsis
  • Prochilodus brevis is a fish species with declining populations, prompting research on improving its reproduction in captivity.
  • The study aimed to identify the optimal sperm-to-oocyte ratio and the best volume of activating solution for fertilization.
  • Results showed that using approximately 928,410 sperm per oocyte and 75.64 mL of activating solution per 2 mL of oocytes maximized fertilization rates, achieving a peak of 51.69%.
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