Picoliter agar droplet breakup in microfluidics meets microbiology application: numerical and experimental approaches.

Droplet microfluidics has provided lab-on-a-chip platforms with the capability of bacteria encapsulation in biomaterials, controlled culture environments, and live monitoring of growth and proliferation. The droplets are mainly generated from biomaterials with temperature dependent gelation behavior which necessitates stable and size-controlled droplet formation within microfluidics. Here, the biomaterial is agar hydrogel with a non-Newtonian response at operating temperatures below 40 °C, the upper-temperature threshold for cells and pathogens.

Dynamics of temperature-actuated droplets within microfluidics.

Characterizing the thermal behavior of dispersed droplets within microfluidic channels is crucial for different applications in lab-on-a-chip. In this paper, the physics of droplets volume during their transport over a heater is studied experimentally and numerically. The response of droplets to external heating is examined at temperature ranges of 25-90 °C and at different flow rates of the dispersed phase respect to the continuous flow.