Comparison of T Lymphocyte Subsets and Natural Killer Lymphocytes in Moderate Versus Severe COVID-19 Patients.

Severe respiratory involvement that follows a process of immune dysregulation and intense cytokine production remains to be the most dreaded complication of Coronavirus Disease-2019 (COVID-19) infection. The aim of this study was to analyze T lymphocyte subsets and natural killer (NK) lymphocytes in moderate and severe cases of COVID-19 infection and assess their significance in disease severity and prognosis. Twenty moderate cases and 20 severe cases of COVID-19 were studied and compared regarding blood picture, biochemical markers, T lymphocyte population subsets, and NK lymphocytes, which were determined by flow cytometric analysis.

Changes in peripheral blood cellular morphology as diagnostic markers for COVID-19 infection.

Introduction: Real-time reverse-transcriptase polymerase chain reaction (RT-PCR) assays were established to detect severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2). However, due to the high rate of false negative results, additional tests as computed tomography (CT) scans of the chest and blood chemistry are required to properly diagnose COVID-19 infection. Abnormal morphological changes of peripheral blood cells as granulocytic dysmorphism and abnormal reactive lymphocytes have been described in some cases.

Effect of mast cell stabilization on angiogenesis in primary and secondary experimental Trichinella spiralis infection.

Background: Mast cells are known to affect the primary and secondary immune responses against parasites, and this effect is partially mediated through the release of pro-angiogenic mediators. The aim of this study was to explore the effect of the mast cell stabilizer (MCS), ketotifen, with and without albendazole, an anti-parasitic prescription medicine, on the inflammatory response against Trichinella spiralis, with the overall aim to investigate its effect on angiogenesis accompanying nurse cell formation.

Methods: The effect of ketotifen and albendazole was explored in eight groups of female BALB/c mice.

Endothelial cell protein C receptor gene 6936A/G and 4678G/C polymorphisms as risk factors for deep venous thrombosis.

Endothelial cell protein C receptor (EPCR) enhances the generation of activated protein C by the thrombin-thrombomodulin complex. A soluble form of EPCR (sEPCR) is present in plasma. Two polymorphisms in the EPCR gene (6936A/G and 4678G/C) have been reported to influence the risk of venous thromboembolism.

Flow cytometric assessment of endothelial and platelet microparticles in preeclampsia and their relation to disease severity and Doppler parameters.

Objective: Platelet (P) and endothelial (E) microparticle (MP) levels increase in preeclampsia. However, their relation to the severity of the disease needs to be clarified. The objectives of this study were to compare the levels of EMP and PMP in severe and mild preeclampsia to healthy gravidas to find possible correlations to severity of the disease, Doppler changes, and complications.

FAS and FAS ligand gene polymorphisms in Egyptian females with preeclampsia.

We aimed to evaluate the association of Fas polymorphism and the Fas ligand with preeclampsia, investigating whether the G 670 Fas gene variant and the Fas Ligand INV2nt 124 G variant had a differential distribution in patients with preeclampsia. The preeclamptic group consisted of 50 pregnant women who developed preeclampsia, while the control group consisted of 50 age-matched pregnant women with uncomplicated pregnancies. Fas and Fas ligand gene polymorphisms were tested using polymerase chain reaction-restriction fragment length polymorphism.

Prevalence of coagulation factor XIII and plasminogen activator inhibitor-1 gene polymorphisms among Egyptian women suffering from unexplained primary recurrent miscarriage.

Recurrent miscarriage (RM) is an obstetric challenge. Polymorphisms of factor XIII (FXIII) and plasminogen activator inhibitor-1 (PAI-1) may cause an imbalance between coagulation and fibrinolysis that can end in RM. The aim of the work was to determine the prevalence of FXIII Val34Leu and PAI-1 4G/5G gene polymorphisms in Egyptian women presenting with unexplained primary first trimester RM.

Association between the monocyte chemoattractant protein-1 -2518G/A gene polymorphism and acute myocardial infarction patients among Egyptian population.

The aim of the present study was to investigate the possible association between the -2518G/A polymorphism of the monocyte chemoattractant protein-1 (MCP-1) gene and acute myocardial infarction (MI) in a sample of the Egyptian population. A total of 30 Egyptian patients with coronary artery disease (CAD) manifested as acute myocardial infarction (MI) for the first time and 25 unrelated healthy control individuals were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The proportion of G/A and G/G genotypes were significantly higher in the acute MI group than the control group (P < 0.