Mitochondrial acyl carrier protein, Acp1, required for iron-sulfur cluster assembly in mitochondria and cytoplasm in Saccharomyces cerevisiae.

Mitochondria perform vital biosynthetic processes, including fatty acid synthesis and iron-sulfur (FeS) cluster biogenesis. In Saccharomyces cerevisiae mitochondria, the acyl carrier protein Acp1 participates in type II fatty acid synthesis, requiring a 4-phosphopantetheine (PP) prosthetic group. Acp1 also interacts with the mitochondrial FeS cluster assembly complex that contains the cysteine desulfurase Nfs1.

Mitochondria function in cytoplasmic FeS protein biogenesis.

Iron‑sulfur (FeS) clusters are cofactors of numerous proteins involved in essential cellular functions including respiration, protein translation, DNA synthesis and repair, ribosome maturation, anti-viral responses, and isopropylmalate isomerase activity. Novel FeS proteins are still being discovered due to the widespread use of cryogenic electron microscopy (cryo-EM) and elegant genetic screens targeted at protein discovery. A complex sequence of biochemical reactions mediated by a conserved machinery controls biosynthesis of FeS clusters.

Functional link hybrid artificial neural network for predicting continuous biohydrogen production in dynamic membrane bioreactor.

Conventional machine learning approaches have shown limited predictive power when applied to continuous biohydrogen production due to nonlinearity and instability. This study was aimed at forecasting the dynamic membrane reactor performance in terms of the hydrogen production rate (HPR) and hydrogen yield (HY) using laboratory-based daily operation datapoints for twelve input variables. Hybrid algorithms were developed by integrating particle swarm optimized with functional link artificial neural network (PSO-FLN) which outperformed other hybrid algorithms for both HPR and HY, with determination coefficients (R) of 0.

Outcome of Open Repair of Thoracoabdominal Aortic Aneurysm in Takayasu Arteritis: A Retrospective Analysis.

Background: Takayasu Arteritis (TA) is an immune mediated arteritis causing inflammation of the aorta and its branches, which can result in aortic aneurysms. Our aim is to describe the outcome of surgical management in these patients who presented with Thoracoabdominal aortic aneurysm (TAAA).

Methods: Between 2003 and 2023, 40 TA patients with TAAA underwent operative repair.

Environmental pollution biomonitoring around a cement factory based on the Air Pollution Tolerance Index of some tree species.

The present study examined the ability of Quercus castaneifolia C.A.M.

Polyhydroxyalkanoate production from rice straw hydrolysate: Insights into feast-famine dynamics and microbial community shifts.

Activated sludge contains a versatile microbiome capable of converting wastes into valuable chemicals like polyhydroxyalkanoates (PHA). This study investigated the influence of repeated feast and famine phases on PHA production as well as the corresponding microbial population dynamics using waste activated sludge (WAS) as inoculum. Hydrolysate derived from rice straw was employed as a substrate for PHA production.

Child Neurology: Progressive Cerebellar Atrophy and Retinal Dystrophy: Clues to an Ultrarare -Related Neurometabolic Diagnosis.

Pathogenic biallelic variants in , which encodes the enzyme mitochondrial aconitase, are associated with the very rare diagnosis of -related infantile cerebellar retinal degeneration (OMIM 614559). We describe the diagnostic odyssey of a 4-year-old female patient with profound global developmental delays, microcephaly, severe hypotonia, retinal dystrophy, seizures, and progressive cerebellar atrophy. Whole-exome sequencing revealed 2 variants in ; c.

Concepts of circular economy for sustainable management of electronic wastes: challenges and management options.

The electronic and electrical industrial sector is exponentially growing throughout the globe, and sometimes, these wastes are being disposed of and discarded with a faster rate in comparison to the past era due to technology advancements. As the application of electronic devices is increasing due to the digitalization of the world (IT sector, medical, domestic, etc.), a heap of discarded e-waste is also being generated.

Essential mitochondrial role in iron-sulfur cluster assembly of the cytoplasmic isopropylmalate isomerase Leu1 in Saccharomyces cerevisiae.

Iron-sulfur (Fe-S) cluster assembly in mitochondria and cytoplasm is essential for cell viability. In the yeast S. cerevisiae, Leu1 [4Fe-4S] is the cytoplasmic isopropylmalate isomerase involved in leucine biosynthesis.

Machine learning in fermentative biohydrogen production: Advantages, challenges, and applications.

Hydrogen can be produced in an environmentally friendly manner through biological processes using a variety of organic waste and biomass as feedstock. However, the complexity of biological processes limits their predictability and reliability, which hinders the scale-up and dissemination. This article reviews contemporary research and perspectives on the application of machine learning in biohydrogen production technology.

Characteristics of the Isu1 C-terminus in relation to [2Fe-2S] cluster assembly and ISCU Myopathy.

Mitochondrial [2Fe-2S] cluster biosynthesis is driven by the coordinated activities of the Iron-Sulfur Cluster (ISC) pathway protein machinery. Within the ISC machinery, the protein that provides a structural scaffold on which [2Fe-2S] clusters are assembled is the ISCU protein in humans; this protein is referred to as the "Scaffold" protein. Truncation of the C-terminal portion of ISCU causes the fatal disease "ISCU Myopathy", which exhibits phenotypes of reduced Fe-S cluster assembly in cells.

Valorization of pretreated waste activated sludge to organic acids and biopolymer.

Polyhydroxybutyrate (PHB) is a natural polyester that may be made by utilizing volatile fatty acids (VFAs) as a substrate. VFA generated by continuous anaerobic fermentation of waste activated sludge (WAS) was fed into bioreactors for PHB synthesis in this work. Series of optimization tests were conducted to increase the biodegradability and hydrolysis of waste activated sludge.

Biocatalytic remediation of industrial pollutants for environmental sustainability: Research needs and opportunities.

An increasing quantum of pollutants from various industrial sector activities represents a severe menace to environmental & ecological balance. Bioremediation is gaining flow globally due to its cost-effective and environment-friendly nature. Understanding biodegradation mechanisms is of high ecological significance.

Thrombotic Thrombocytopenic Purpura: A Tale of Two Cases.

Microangiopathic hemolytic anaemia, thrombocytopenia, renal failure, neurologic abnormalities, and fever form the pentad of thrombotic thrombocytopenic purpura (TTP). Early diagnosis is crucial because TTP responds well to plasmapheresis therapy but is associated with substantial mortality if left untreated. A substantial percentage of patients with TTP used to die from systemic microvascular thrombosis in the brain and the heart.

Recent advances in computational fluid dynamics (CFD) modelling of photobioreactors: Design and applications.

The development of photobioreactor is important for sustainable production of renewable fuels, wastewater treatment and CO fixation. For the design and scale-up of a photobioreactor, CFD can be used as an indispensable tool. The present study reviews the recent status of computational flow modelling of various types of photobioreactors, involving fluid dynamics, light transport, and algal growth kinetics.

Inhibition of mitochondrial LonP1 protease by allosteric blockade of ATP binding and hydrolysis via CDDO and its derivatives.

The mitochondrial protein LonP1 is an ATP-dependent protease that mitigates cell stress and calibrates mitochondrial metabolism and energetics. Biallelic mutations in the LONP1 gene are known to cause a broad spectrum of diseases, and LonP1 dysregulation is also implicated in cancer and age-related disorders. Despite the importance of LonP1 in health and disease, specific inhibitors of this protease are unknown.

Production of microalgae with high lipid content and their potential as sources of nutraceuticals.

In the current global scenario, the world is under a serious dilemma due to the increasing human population, industrialization, and urbanization. The ever-increasing need for fuels and increasing nutritional problems have made a serious concern on the demand for nutrients and renewable and eco-friendly fuel sources. Currently, the use of fossil fuels is creating ecological and economic problems.

Roles of host mitochondria in the development of COVID-19 pathology: Could mitochondria be a potential therapeutic target?

The recent emergence of severe acute respiratory syndrome-Corona Virus 2 (SARS-CoV-2) in late 2019 and its spread worldwide caused an acute pandemic of Coronavirus disease 19 (COVID-19). Since then, COVID-19 has been under intense scrutiny as its outbreak led to significant changes in healthcare, social activities, and economic settings worldwide. Although angiotensin-converting enzyme-2 (ACE-2) receptor is shown to be the primary port of SARS-CoV-2 entry in cells, the mechanisms behind the establishment and pathologies of COVID-19 are poorly understood.

Dark fermentation: Production and utilization of volatile fatty acid from different wastes- A review.

Volatile fatty acids (VFAs) are the building blocks of the chemical industry, and they are the primary contributors to the planet's organic carbon cycle. VFA production from fossil fuels (mostly petroleum) is unsustainable, pollutes the environment, and generates greenhouse gases. As a result of these issues, there is a pressing need to develop alternate sources for the long-term generation of VFAs via anaerobic digestion.

Determination of landfill gas generation potential from lignocellulose biomass contents of municipal solid waste.

The presence of heat, methane (CH) and oxygen in landfill sub-surface causes initiation of spontaneous waste ignition posing severe environmental impacts. A municipal solid waste (MSW) reactor (trough) was designed to monitor landfill gases (LFGs) i.e.

Intron exon boundary junctions in human genome have in-built unique structural and energetic signals.

Precise identification of correct exon-intron boundaries is a prerequisite to analyze the location and structure of genes. The existing framework for genomic signals, delineating exon and introns in a genomic segment, seems insufficient, predominantly due to poor sequence consensus as well as limitations of training on available experimental data sets. We present here a novel concept for characterizing exon-intron boundaries in genomic segments on the basis of structural and energetic properties.

Gargantuan internal carotid artery aneurysm-a surgical challenge.

Extracranial carotid artery aneurysms are a rarely reported entity. Here, we describe an unusually large internal carotid artery aneurysm in a 76-year-old female, with progressive enlargement and history of thromboembolic event. She was managed successfully with an open repair and common carotid artery to internal carotid artery bypass.

Role of microbial community and metal-binding proteins in phytoremediation of heavy metals from industrial wastewater.

This review illustrated the role of metal-binding proteins (MBPs) and microbial interaction in assisting the phytoremediation of industrial wastewater polluted with heavy metals. MBPs are used to increase the accumulation and tolerance of metals by microorganisms via binding protein synthesis. Microbes have various protection mechanisms to heavy metals stress like compartmentalization, exclusion, complexity rendering, and the synthesis of binding proteins.

Response surface methodology and artificial neural network modelling for enhancing maturity parameters during vermicomposting of floral waste.

In this study, the mixture of floral waste and cattle dung in different proportions was utilised to convert into vermicompost using earthworm Eisenia fetida. In the design of the experiment, the optimum amount of floral waste (1325 g) and cattle dung (500 g) was obtained for vermicompost using central composite design (CCD) and compared with the output of artificial neural network (ANN). The optimum proportions of vermicompost showed pH of 7.