Publications by authors named "Ashton M Kelly"
Article Synopsis
- Epstein-Barr virus (EBV) is linked to classical Hodgkin lymphoma (cHL), but the role of antibodies against EBV in cHL patients isn't fully understood, prompting a study to investigate this connection.
- Researchers conducted a custom protein microarray study comparing antibody responses in EBV-positive cHL patients from East Asia with healthy controls, discovering a specific antibody profile unique to this population.
- The study found that a majority of these antibodies were also associated with cHL in a separate European population, indicating that certain EBV antibodies may serve as reliable biomarkers for EBV-positive cHL across different demographics.
Article Synopsis
- The study evaluates the stability of twelve commonly used reference genes in RTqPCR analysis following Plasmodium yoelii sporozoite challenge and immunization in mice.
- It found significant expression changes in six of these genes due to the parasite challenge or immunization, indicating that not all reference genes are suitable for this type of analysis.
- SDHA and TBP were identified as stable reference genes, leading to the development of a robust RTqPCR protocol that can improve the consistency and reliability of malaria vaccine efficacy studies across different research settings.
Whole-blood-derived transcriptional profiling is widely used in biomarker discovery, immunological research, and therapeutic development. Traditional molecular and high-throughput transcriptomic platforms, including molecular assays with quantitative PCR (qPCR) and RNA-sequencing (RNA-seq), are dependent upon high-quality and intact RNA. However, collecting high-quality RNA from field studies in remote tropical locations can be challenging due to resource restrictions and logistics of post-collection processing.
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- Current immune assays are sensitive but require a lot of cells and expensive reagents, making them impractical for large-scale testing.
- A new RT-qPCR-based high-throughput screening assay has been developed, which significantly reduces the need for cell numbers and lowers costs by almost 90% while maintaining sensitivity.
- This optimized assay can accurately measure immune responses using as few as 50,000 PBMCs, making it ideal for situations where samples are limited and budgets are tight.