Publications by authors named "Ashraf Hesham Barkawi"
The present study was designed to establish a suitable alternative approach to mitigate the adverse effect of high culture temperature on in vitro embryo development and the related molecular response in buffalo. Pre-cultured granulosa cells (GCs) were used as a monolayer during in vitro embryo culture until day 8 (day of fertilization = D0). Post fertilization, presumptive embryos were randomly assigned into two culture conditions: embryos cultured in the presence of GCs monolayer under normal culture temperature (N: 38.
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- - The study examined how different oxygen levels affect the quality of buffalo cumulus-oocyte complexes (COCs), analyzing factors like cellular structure, mitochondrial activity, and gene expression in matured oocytes.
- - Results showed that low oxygen tension (5%) was beneficial for oocyte quality, leading to better mitochondrial activity and gene expression, while high oxygen tension (20%) negatively impacted lower quality oocytes (BCB-), increasing reactive oxygen species levels.
- - The findings suggest that maintaining a low oxygen environment is crucial for nurturing high-quality buffalo oocytes, especially in promoting nuclear maturation and metabolic activity.