Intraspecific Diversity of Populations Isolated from Cystic Fibrosis Respiratory Infections.

Unlabelled: Chronic bacterial infections are often polymicrobial, comprising multiple bacterial species or variants of the same species. Because chronic infections may last for decades, they have the potential to generate high levels of intraspecific variation through within-host diversification over time, and the potential for superinfections to occur through the introduction of multiple pathogen populations to the ongoing infection. Traditional methods for identifying infective agents generally involve isolating one single colony from a given sample, usually after selecting for a specific pathogen or antibiotic resistance profile.

Experimentally evolved shows increased survival in the presence of by acquiring mutations in the amino acid transporter, GltT.

When cultured together under standard laboratory conditions has been shown to be an effective inhibitor of . However, and are commonly observed in coinfections of individuals with cystic fibrosis (CF) and in chronic wounds. Previous work from our group revealed that isolates from CF infections are able to persist in the presence of strain PAO1 with a range of tolerances with some isolates being eliminated entirely and others maintaining large populations.

Experimentally Evolved Survives in the Presence of by Acquiring Mutations in the Amino Acid Transporter, GltT.

and are the most common bacterial pathogens isolated from cystic fibrosis (CF) related lung infections. When both of these opportunistic pathogens are found in a coinfection, CF patients tend to have higher rates of pulmonary exacerbations and experience a more rapid decrease in lung function. When cultured together under standard laboratory conditions, it is often observed that effectively inhibits growth.

Species-Wide Phylogenomics of the Staphylococcus aureus Operon Revealed Convergent Evolution of Frameshift Mutations.

Staphylococcus aureus is a prominent nosocomial pathogen that causes several life-threatening diseases, such as pneumonia and bacteremia. S. aureus modulates the expression of its arsenal of virulence factors through sensing and integrating responses to environmental signals.

Genotypic and Phenotypic Diversity of Staphylococcus aureus Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with Pseudomonas aeruginosa.

has recently overtaken as the most commonly recognized bacterial pathogen that infects the respiratory tracts of individuals with the genetic disease cystic fibrosis (CF) in the United States. Most studies of in CF patient lung infections have focused on a few isolates, often exclusively laboratory-adapted strains, and how they are killed by Less is known about the diversity of CF patient lung isolates in terms of both their virulence and their interaction with To begin to address this gap, we recently sequenced 64 clinical isolates and a reference isolate, JE2. Here, we analyzed the antibiotic resistance genotypes, sequence types, clonal complexes, types, types, and presence/absence of other known virulence factor genes of these isolates.