Publications by authors named "Ashley DeAguero"
Article Synopsis
- The text discusses a new platform for creating antibodies with high effectiveness and diversity, building on a previously established single-chain fragment (scFv) library that used clinical antibodies as a base.
- This advancement involves using Fab phage display followed by scFab yeast display, resulting in better expression balance of antibody chains and improved conversion rate to IgG antibodies, combining the benefits of scFvs and Fabs.
- A quality-controlled Fab phage library was developed, showcasing its potential to produce a diverse range of binding scFabs with high efficiency, ultimately streamlining the path for these antibodies to become viable therapeutic options.
Activity of the myogenic regulatory protein myocyte enhancer factor-2 (MEF2) is modulated by post-translational modification. We investigated the in vivo phosphorylation of MEF2, and identified serine 98 (S98) as a phosphorylated residue. Phospho-mimetic (S98E) and phospho-null (S98A) isoforms of MEF2 did not differ from wild-type in their activity in vitro, so we used CRISPR/Cas9 to generate an S98A allele of the endogenous gene.
View Article and Find Full Text PDFCDR: complementarity determining region; FACS: fluorescence-activated cell sorting; k: association rate; k: dissociation rate; K: dissociation constant; scFv: single-chain variable fragment; SPR: surface plasmon resonance.
View Article and Find Full Text PDFSerum response factor (SRF) has an established role in controlling actin homeostasis in mammalian cells, yet its role in non-vertebrate muscle development has remained enigmatic. Here, we demonstrate that the single SRF ortholog, termed Blistered (Bs), is expressed in all adult muscles, but Bs is required for muscle organization only in the adult indirect flight muscles. Bs is a direct activator of the flight muscle actin gene , via a conserved promoter-proximal binding site.
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