Effect of Various Surface Treatments of Zirconia on its Adhesive Properties to Dentin: An Study.

Aim: To assess the effectiveness of various surface treatments and adhesives on the bond strength of zirconia-based ceramic to dentin.

Materials And Methods: Eighty samples of zirconia were subjected to the four-surface treatment protocols (sandblasting, 48% hydrofluoric acid (HF), 48% hydrofluoric acid + 70% nitric acid (HNO) and no treatment (control) following which the samples from each group were subdivided into two subgroups ( = 10) based on the resin cement employed for cementation (RelyX U200 and G-Cem Linkforce). The bonded specimens were subjected to shear stress to measure the bond strength using Universal testing machine.

Chromosome organization shapes replisome dynamics in Caulobacter crescentus.

DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial species, driving the hypothesis that a physical linker may tether them together. However, replisome splitting has also been reported in many of the same species, leaving the principles behind replisome organization a long-standing puzzle.

Mechanistic insight into the repair of C8-linked pyrrolobenzodiazepine monomer-mediated DNA damage.

Pyrrolobenzodiazepines (PBDs) are naturally occurring DNA binding compounds that possess anti-tumor and anti-bacterial activity. Chemical modifications of PBDs can result in improved DNA binding, sequence specificity and enhanced efficacy. More recently, synthetic PBD monomers have shown promise as payloads for antibody drug conjugates and anti-bacterial agents.

Comparative Evaluation of Root Canal Centering Ability of ProTaper, Mtwo, WaveOne, and Reciproc Using Cone-beam Computed Tomography: Study.

Aim: To compare the centering ability of rotating (ProTaper and Mtwo) and reciprocating (WaveOne and Reciproc) file systems using cone-beam computed tomography (CBCT).

Materials And Methods: Eighty extracted human mandibular molars with curvature within 15-45° were selected and randomly divided into four groups ( = 20): group I (ProTaper), group II (Mtwo), group III (WaveOne), and group IV (Reciproc). The selected teeth were arranged in a template, and pre-instrumentation and post-instrumentation CBCT scans were taken using Kodak Carestream CS 9300 machine.

Evaluation of the Antimicrobial Efficacy of Oil, Oil and 5% Sodium Hypochlorite Against Biofilm Formed on Tooth Substrate.

Context: The usual cause of nonfulfillment of endodontic therapy is the persistence of microorganisms in the root canal system due to ineffective disinfection. is one of the most prevalently isolated microorganisms following a failure in root canal treatments. Sodium hypochlorite is among the most effectively used irrigant solutions but has many shortcomings.

Coordination between nucleotide excision repair and specialized polymerase DnaE2 action enables DNA damage survival in non-replicating bacteria.

Translesion synthesis (TLS) is a highly conserved mutagenic DNA lesion tolerance pathway, which employs specialized, low-fidelity DNA polymerases to synthesize across lesions. Current models suggest that activity of these polymerases is predominantly associated with ongoing replication, functioning either at or behind the replication fork. Here we provide evidence for DNA damage-dependent function of a specialized polymerase, DnaE2, in replication-independent conditions.

Prevalence of Dental Caries in Adult South Indian Population in Association with Dietary Pattern: A Comparative Study.

Background: Dental caries is considered to be one of the most prevalent dental diseases among humans. It involves cycles of demineralization and remineralization. The etiology and pathogenesis of dental caries are multifactorial.

Visualizing mutagenic repair: novel insights into bacterial translesion synthesis.

DNA repair is essential for cell survival. In all domains of life, error-prone and error-free repair pathways ensure maintenance of genome integrity under stress. Mutagenic, low-fidelity repair mechanisms help avoid potential lethality associated with unrepaired damage, thus making them important for genome maintenance and, in some cases, the preferred mode of repair.

A comparative study of the evolution of cellobiose utilization in Escherichia coli and Shigella sonnei.

The chb operon of Escherichia coli is involved in the utilization of chitooligosaccharides. While acquisition of two classes of mutations leading to altered regulation of the chb operon is necessary to confer the ability to utilize the glucose disaccharide cellobiose to wild-type strains of E. coli, in the closely related organism Shigella sonnei, Cel mutants arise relatively faster, requiring only a single mutational event.

Evaluation and comparison of the microhardness of enamel after bleaching with fluoride free and fluoride containing carbamide peroxide bleaching agents and post bleaching anticay application: An in vitro study.

Aims And Objectives: The purpose of the study was to evaluate and compare the microhardness of enamel after the application of anticay on bleached enamel with fluoride containing and fluoride free bleaching agent.

Materials And Methods: Twenty freshly extracted teeth decoronated and divided mesiodistally into two halves were randomly divided into five groups with 10 samples in each group. The enamel surface was treated as follows: Group 1 - no treatment, Group 2 - fluoride free bleaching agent, Group 3 - fluoride containing bleaching agent, and Group 4 - fluoride free bleaching agent followed by anticay application.

Microleakage evaluation of Silorane-based composite and methacrylate-based composite in class II box preparations using two different layering techniques: an in vitro study.

Aim: The aim of this study was to compare the microleakage in Class II box preparations with the gingival margin above and below the cemento-enamel junction (CEJ) restored with Silorane composite and methacrylate composite using two different layering techniques.

Materials And Methods: Standardized box preparations (mesial box 1 mm above the CEJ and distal box 1 mm below the CEJ) were prepared in 60 upper premolars. The teeth were randomly divided into four groups containing 15 samples each; Group I: Restored with a Silorane composite using an oblique layering technique, Group II: Restored with Silorane composite using a vertical layering technique, Group III: Restored with methacrylate composite using the oblique layering technique, and Group IV: Restored with methacrylate composite using the vertical layering technique.

Multifocal granular cell tumor presenting as an esophageal stricture.

Introduction: Granular cell tumors are uncommonly found in the gastrointestinal tract with slow progression and are usually benign though they may have propensity for malignant transformation. Initially attributed to neuronal origin through immunohistochemistry, there has been controversy with increasing reports of granular cell tumors of non-neural origin.

Case Report: We report a case of multifocal granular cell tumor involving the esophagus and stomach in a young female with history of dysphagia for 9 years with worsening symptoms.