Publications by authors named "Ash W J Tie"
Methods Mol Biol
July 2024
Article Synopsis
- Genome editing tools like CRISPR systems, particularly CRISPR/Cas9, show promise for safe and customizable cancer therapies by targeting transcriptional abnormalities with minimal off-target effects.
- * Despite advances, challenges remain in effectively delivering these large dCas9 fusion proteins into cancer cells, including concerns about immune responses and the complexities of viral delivery methods.
- * The emergence of mRNA vaccine technology offers new possibilities for CRISPR/dCas9 delivery, with lipid nanoparticles (LNPs) being explored as a nonviral alternative due to their scalability and lower immunogenicity.
