Publications by authors named "Ash Maroof"
Objectives: A number of immune populations have been implicated in psoriatic arthritis (PsA) pathogenesis. This study used mass cytometry (CyTOF) combined with transcriptomic analysis to generate a high-dimensional dataset of matched PsA synovial fluid (SF) and blood leucocytes, with the aim of identifying cytokine production ex vivo in unstimulated lymphoid and myeloid cells.
Methods: Fresh SF and paired blood were either fixed or incubated with protein transport inhibitors for 6 hours.
Psoriatic arthritis (PsA) is a debilitating immune-mediated inflammatory arthritis of unknown pathogenesis commonly affecting patients with skin psoriasis. Here we use complementary single-cell approaches to study leukocytes from PsA joints. Mass cytometry demonstrates a 3-fold expansion of memory CD8 T cells in the joints of PsA patients compared to peripheral blood.
View Article and Find Full Text PDFThe pro-inflammatory cytokine IL-17A has been implicated in the immunopathology of inflammatory arthritis. IL-17F bears 50% homology to IL-17A and has recently been suggested to play a role in inflammation. We investigated the induction and cytokine profile of IL-17F CD4 T cells, and how IL-17F may contribute to inflammation.
View Article and Find Full Text PDFArticle Synopsis
- IL-17A is crucial for inflammation in immune diseases, but the role of IL-17F is less understood; the study hypothesizes that both contribute to chronic inflammation and that targeting both may be more effective.
- Preclinical tests showed that IL-17F triggers similar inflammatory reactions as IL-17A, and the clinical trial of bimekizumab (a drug neutralizing both IL-17A and IL-17F) demonstrated better inflammation control compared to targeting IL-17A alone.
- Results from the trial indicate that dual inhibition significantly improved patient outcomes in psoriatic arthritis by reducing symptoms in skin and joints, supporting the importance of IL-17F in inflammatory processes.