Trace metal exposure of soil bacteria depends on their position in the soil matrix.

Micropores and biofilms of soils may protect bacteria against chemical stress, predation, and competition phenomena, explaining the great diversity and robustness of soil microbial communities and functions. We used sequential dispersion/density gradient centrifugation to separate free and loosely attached cells (FLA) from strongly attached cells (SA). The two fractions of the soils communities were investigated along a Zn and Cd pollution gradient, and the pollution-induced trace metal community tolerance (PICT) for SA and FLA was analyzed.

Speciation of Cd and Zn in contaminated soils assessed by DGT-DIFS, and WHAM/Model VI in relation to uptake by spinach and ryegrass.

A pot experiment was carried out to investigate the impact of Cd and Zn extractability in soil and speciation in pore water of industrial contaminated soils, on metal concentration in a metal sensitive species like spinach (Spinacia oleracea) and a more metal tolerant species like Italian ryegrass (Lolium multiflorum). For chemical speciation of Cd and Zn in pore water, WHAM/Model VI version 6.0 was used.

Plant uptake of cadmium-109 and zinc-65 at different temperature and organic matter levels.

The uptake of 109Cd and 65Zn and their stable isotopes by ryegrass (Lolium multiflorum Lam.), grown on two different soil types, was investigated in climatically controlled growth chambers at 9 and 21 degrees C. The soils were treated with 0 and 4% organic matter (pig [Sus scrofa] manure) and spiked with 109Cd and 65Zn before sowing.