Publications by authors named "Asep Ridwanuloh"
Article Synopsis
- * Traditional methods for studying DNA methylation are invasive, so this study explores using buccal (cheek) cells as a less invasive alternative to collect biomarkers related to stunting.
- * Using adaptive sampling from Oxford Nanopore Technology, researchers found that stunted children had lower levels of 5-methylcytosine and identified specific gene loci on chromosome 21 that are differentially methylated and previously unassociated with stunting.
Article Synopsis
- In Indonesia, early-onset colorectal cancer (EOCRC) occurs more frequently in those under 50, potentially linked to a higher incidence of Lynch syndrome (LS) among CRC patients.
- A study at Dr. Sardjito General Hospital examined 244 samples for mutations in KRAS and PIK3CA, finding significant associations between these mutations, microsatellite instability (MSI) status, and Lynch syndrome.
- Results indicated that KRAS mutations correlate with right-sided colorectal cancer and higher histology grades, while PIK3CA mutations link to female patients and lower tumor-infiltrating lymphocyte levels; however, neither mutation significantly impacted overall survival.
Introduction: COVID-19 is an emerging infectious disease that remains to be further investigated.
Case Report: Here, we describe a case of COVID-19 in an octogenarian woman with comorbidities who slowly recovered during hospitalization, but died due to sudden cardiac death after 2 weeks of hospitalization. Her nasopharyngeal and anal swabs returned positive for SARS-CoV-2 by RT-PCR on day 7 of hospitalization.
Whole-genome sequencing (WGS) has played a significant role in understanding the epidemiology and biology of SARS-CoV-2 virus. Here, we investigate the use of SARS-CoV-2 WGS in Southeast and East Asian countries as a genomic surveillance during the COVID-19 pandemic. Nottingham-Indonesia Collaboration for Clinical Research and Training (NICCRAT) initiative has facilitated collaboration between the University of Nottingham and a team in the Research Center for Biotechnology, National Research and Innovation Agency (BRIN), to carry out a small number of SARS-CoV-2 WGS in Indonesia using Oxford Nanopore Technology (ONT).
View Article and Find Full Text PDFBackground: Indonesia is one of the Southeast Asian countries with high case numbers of COVID-19 with up to 4.2 million confirmed cases by 29 October 2021. Understanding the genome of SARS-CoV-2 is crucial for delivering public health intervention as certain variants may have different attributes that can potentially affect their transmissibility, as well as the performance of diagnostics, vaccines, and therapeutics.
View Article and Find Full Text PDFThere is about three times higher incidence of young patients <50 years old with colorectal cancer, termed EOCRC, in Indonesia as compared to Europe, the UK and USA. The aim of this study was to investigate the frequency of Lynch Syndrome (LS) in Indonesian CRC patients. The previously described Nottingham Lynch Syndrome Test (N_LyST) was used in this project.
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- Lung cancer patients with EGFR gene mutations receive treatment using tyrosine kinase inhibitors (TKIs), and the study aimed to assess methods for detecting these mutations in cell-free DNA before and after treatment.
- Using various techniques (PCR-HRM, RFLP, and direct sequencing), researchers analyzed samples from 116 treatment-naïve patients and found that while 100% of cfDNA samples showed EGFR genotypes, the sensitivity of plasma testing for mutations was relatively low compared to cytological samples.
- After three months of TKI treatment, new resistance mutations were detected in some patients' plasma, suggesting that these tests can identify treatment resistance early on despite low sensitivity in detecting certain mutations.
Indonesia has the highest cigarette consumption in the world. We explored the clinical impact of smoking on the prevalence of and mutations and survival in this prospective study. 143 treatment naive lung cancer patients were recruited from Persahabatan Hospital, a national tertiary hospital.
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