Mono- and sesquiterpenoid fingerprinting: A powerful and streamlined solution for pine nut authentication.

This study proposes a novel authentication method for pine nut geographical and botanical origin, using mono- and sesquiterpene fingerprints (extracted ion chromatograms from specific ions) analysed via solid-phase microextraction coupled with gas chromatography-mass spectrometry, combined with chemometrics (partial least squares - discriminant analysis). It was tested on 253 samples from China, Russia (major producers of Pinus koraiensis and Pinus sibirica), Spain and Turkey (supplying Pinus pinea), across harvest years. The method achieved 100 % accuracy in external validation when distinguishing Spanish from non-Spanish pine nuts, and 99 % accuracy in differentiating Pinus pinea samples from two distinct Spanish regions.