SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins.

Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements.

Challenges of the Human Proteome Project: 10-Year Experience of the Russian Consortium.

This manuscript collects all the efforts of the Russian Consortium, bottlenecks revealed in the course of the C-HPP realization, and ways of their overcoming. One of the main bottlenecks in the C-HPP is the insufficient sensitivity of proteomic technologies, hampering the detection of low- and ultralow-copy number proteins forming the "dark part" of the human proteome. In the frame of MP-Challenge, to increase proteome coverage we suggest an experimental workflow based on a combination of shotgun technology and selected reaction monitoring with two-dimensional alkaline fractionation.

[Automatic detection of ventricular and supraventricular wide QRS arrhythmias using complex of morphological criteria and algorithms].

Aim: The aim of study is a detection of ventricular and supraventricular wide QRS arrhythmias using complex of morphological criteria and algorithms by method of automatic analysis.

Materials And Methods: For 100 patients (m/f - 61/39, Me (min; max) - 44.5 (10; 85) years) of researched group the analysis of 14306 single wide ectopic complexes (QRS 120-230 ms) has been done.

[The state of membranes of mononuclear leukocytes in patients with tuberculosis of lungs.].

The sampling of examined patients comprised 308 individuals with medication sensitive (or with absence of data about resistance) tuberculosis of lungs without decay of pulmonary tissue with purpose to detect characteristics of alteration of content and structural organization of monocytic membranes before treatment and also against the background of application of chemotherapy according 1 standard regimen. The data of clinical radiologic examination of patients, bacterioscopy, molecular genetic analysis and analysis of phospholipid spectrum of membranes of mononuclears of peripheral blood were used. To detect particular classes of phospholipids the thin-layer flow chromatography was applied.

[Registration of the protein in the serum with a field-effect nanotransistor biosensor].

A method for detection of cancer-associated protein D-NFATc1 in serum using nanowire (NW) biosensor based on field-effect nanotransistor is developed. Field-effect nanotransistor was fabricated on the basis of «silicon-on-insulator» structures. For the biospecific detection of target protein, the NW surface was modified with aptamers against the target protein.

SOI nanowire for the high-sensitive detection of HBsAg and α-fetoprotein.

Silicon-on-isolator-nanowires (SOI-NWs) were used for the label-free, real-time biospecific detection of the hepatitis B marker HBsAg and cancer marker α-fetoprotein (AFP). Specific protein-protein recognition was carried out using individual NWs that were functionalized with antibodies. To solve the problem of non-specific binding of target protein molecules to the sensor element the use of a reference NW with immobilized antibodies against non-target proteins was proposed.

Quantum interferential Y-junction switch.

We report the observation of the Fermi energy controlled redirection of the ballistic electron flow in a three-terminal system based on a small (100 nm) triangular quantum dot defined in a two-dimensional electron gas (2DEG). Measurement shows strong large-scale sign-changing oscillations of the partial conductance coefficient difference G(21) - G(23) on the gate voltage in zero magnetic field. Simple formulas and numerical simulation show that the effect can be explained by quantum interference and is associated with weak asymmetry of the dot or inequality of the ports connecting the dot to the 2DEG reservoirs.

Gene-centric view on the human proteome project: the example of the Russian roadmap for chromosome 18.

During the 2010 Human Proteome Organization Congress in Sydney, a gene-centric approach emerged as a feasible and tractable scaffold for assemblage of the Human Proteome Project. Bringing the gene-centric principle into practice, a roadmap for the 18th chromosome was drafted, postulating the limited sensitivity of analytical methods, as a serious bottleneck in proteomics. In the context of the sensitivity problem, we refer to the "copy number of protein molecules" as a measurable assessment of protein abundance.

Molecular-beam epitaxy-grown Si whisker structures: morphological, optical and electrical properties.

Scanning electron microscopy, spectroscopic ellipsometry, and current-voltage and current-temperature measurements were employed to characterize nanowhisker structures grown by molecular-beam epitaxy on Si(111) substrates. Small clusters of gold deposited on the Si surface were used as the seeds for nanowhisker growth. The diameter of grown nanowhiskers and their length ranged from 70 to 200 nm and from 580 to 890 nm, respectively.

[Long-term results of treatment in patients with pulmonary tuberculosis, by using main bronchial occlusion, impact of pulmonary hemodynamics].

The outcomes of treatment were analyzed in 108 patients with disseminated and complicated pulmonary tuberculosis. The patient's mean age was 38.3 +/- 4.

Application of ultrahigh vacuum reflection electron microscopy for the study of clean silicon surfaces in sublimation, epitaxy, and phase transitions.

The construction and performance of an ultrahigh vacuum reflection electron microscope (UHV REM) on the base of a transmission electron microscope with top entry stage are described. Some results of in situ study of structural transformations on clean silicon surfaces during sublimation, surface phase transitions, and initial stages of epitaxial growth are shown.

[The attitude of medical institute students to cancer patients].

One hundred and twenty-eight fourth-year and 106 fifth-year medical students were questioned to evaluate their attitude to cancer patients. After taking a course in oncology, 11.3% of students considered cancer "contagious" or "likely to be contagious" and half the students considered cancer prevention "ineffective" or "likely to be ineffective".

[The rehabilitation of breast cancer patients].

Immediate and long-term anatomic, functional, psychologic and social results of radical treatment of breast cancer were assessed in 2 groups of patients who had either undergone or not undergone rehabilitation. The study showed that purposeful rehabilitative treatment and organizational measures assured a decrease in invalidism rate in working age patients from 75 to 43.6% and better quality of life.