Microcalorimetric studies on metabolism of hepatic tissue. II. Measurements of ischemic tissue.

Biopsies of ischemic livers demonstrated a significantly higher rate of heat production when incubated in Leibowitz L-15 medium compared to incubation in modified Eagle's solution (P less than 0.01). A maximal reduction of 25% in the rate of heat production was observed after 60 min of induced ischemia.

Microcalorimetric studies on metabolism of hepatic tissue. I. A methodological study of normal tissue.

In the present study, the heat production of liver biopsies (5-8 mg) was measured by a microcalorimetric technique. Tissue incubated in Leibowitz L-15 medium (L-15) showed a higher metabolic rate compared to tissue incubated in a medium without substrate 2.8 microW/mg and 1.

Rounding of cultured human carcinoid tumor cells by forskolin.

A primary culture of carcinoid cells obtained from a metastasized brain tumor of a 30 year old man was established. Rounding of carcinoid cells was induced by the addition of 20 microM forskolin or 1 mM dibutyryl cyclic AMP to the culture medium. The cyclic AMP content in carcinoid cells was increased thirtyfold by the addition of 20 microM forskolin.

Effect of subculturing on expression of a cell-surface protein antigen by Streptococcus mutans.

Two freshly isolated strains, Xc and Yc, of Streptococcus mutans serotype c from human dental plaque were subcultured 100 times in Brain Heart Infusion broth. The cell-surface hydrophobicity of strain Xc markedly decreased after subculturing 60 times, but that of strain Yc remained unaltered. Radioimmunoassay showed a close correlation between surface hydrophobicity and the amount of a cell-surface protein antigen (PAc) of Mr 190,000.

[A clinical study of the radiosensitization of aclarubicin].

Sixty six patients with an advanced or a relapsed cancer have been treated with a combination of radiation and aclarubicin (ACR). The average irradiation dose was 45 +/- 15 Gy, and the combined total dose of ACR ranged from 130 to 260 mg, depending upon the following differing schedules: 10 mg x 3 or 5/w, 20 mg x 2 or 3/w and 20 mg x 2/d x 2/w. The tumor response attained was 70%, including 30% who achieved a complete response.

Retarding liver cancer growth in the rat by transient repeated hepatic dearterialization.

The effect of repeated ischemic episodes to experimental liver tumors is studied in a group of inbred Wistar-Furth rats. A vascular occluder model was developed specially for the purpose of delivering intermittent compressions to the hepatic artery in the rat. With five daily 1-hr occlusions of the hepatic artery, rats benefited from significantly reduced tumor growth rates compared with controls that underwent sham operation (P less than 0.

Acute ischemic liver failure in the rat: a reproducible model not requiring portal decompression.

We report a model of acute ischemic liver failure which does not require temporary or permanent portal decompression. The model is induced by segmental ischemia of the median and left lateral lobes for 100 min by a vascular clamp applied on the afferent vessels. Declamping is followed by resection of the nonischemic right lateral and caudate lobes.

Preparation of colloidal gold for staining proteins electrotransferred onto nitrocellulose membranes.

This paper describes a simple method of preparing colloidal gold for staining protein blots. Colloidal gold was prepared from 0.005 or 0.

Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.

To clarify the pathogenesis of insulitis in the nonobese diabetic (NOD) mouse, an animal model for human insulin-dependent diabetes mellitus, T-lymphocyte-depleted NOD mice (B mice) were adoptively transferred with spleen and lymph node cells from cyclophosphamide-treated NOD mice after separating the cells with monoclonal antibodies against various T-lymphocyte surface antigens plus complement. Light-microscopic and immunohistochemical studies were also performed to investigate the lymphocytic infiltrations. The incidence of insulitis detected in B mice was much lower when compared with that of the lesion naturally occurring in the NOD mouse.

beta-Hexosaminidase in plasma and liver after partial hepatectomy in normal and cirrhotic rats.

The liver and plasma level of the lysosomal enzyme beta-hexosaminidase was analyzed in partially (about 70%) hepatectomized rats with normal liver or carbon tetrachloride (CCl4)-induced cirrhosis. The enzyme level of the liver did not show marked changes after partial hepatectomy in either normal or cirrhotic rats. In contrast, the plasma basal level was significantly higher in cirrhotic rats and increased to a peak at 6 h, followed by a decrease to a minimum level 24 h after operation.

Ultrastructure of extracellular polysaccharides produced by serotype c Streptococcus mutans.

The ultrastructure of extracellular polysaccharides produced in colonies by two clinical isolates and that of a nitrosoguanidine-induced mutant of serotype c Streptococcus mutans with different polysaccharide-synthesizing abilities were compared electron-microscopically. A large amount of polysaccharide was produced from sucrose by colonies of typical serotype c strain MT8148R and a clinical variant MT6801R with an enhanced fructan-synthesizing ability. Transmission electron-microscopy (TEM) revealed that the polysaccharides consisted of three structural components, i.

Serological relationships between serotype-III Streptococcus sanguis and Lancefield group-H streptococci.

Certain strains of Streptococcus sanguis and group-H streptococci have been shown to have similar physiological properties and serological specificities. Serological studies revealed that serotype-III S. sanguis shared a common antigen with the so-called "British" group-H streptococci, but not with the "American" group-H streptococci.

[Prognosis of 5-year survivors of esophageal cancer treated with radiation and chemotherapy].

Seventeen 5-year survivors, treated with a combination of radiation and chemotherapy, were followed up from 5 to 10 years. Out of them, 10 cases died within 10 years after the completion of radiotherapy. Late local recurrence and remote metastasis of esophageal cancer were observed in 3 cases, and other malignancies of the stomach, lung and esophagus were found in 3 other cases.

[Analysis of 5-year survivors of esophageal cancer treated with radiation and chemotherapy].

From 1967 to 1980, 240 cases of esophageal cancer, consisting of 153 curative and 87 palliative cases, were treated with a combination of radiation and chemotherapy. The five-year survival rate in 124 curative cases given a dose of more than 50 Gy was 12.9%, and 17 cases survived more than 5 years.

Control of traumatic liver hemorrhage in the cirrhotic rat by intraportal infusion of norepinephrine.

The effect of intraportal infusion of norepinephrine (NE) on primary hemostasis in the cirrhotic rat was investigated at standardized liver trauma. Cirrhosis was induced by simultaneous administration of increasing amounts of carbontetrachloride (CCl4) and phenobarbitone. Infusion of norepinephrine took place after cannulation of the gastroduodenal vein.

Sucrose-dependent cell adherence and cariogenicity of serotype c Streptococcus mutans.

Four strains of serotype c Streptococcus mutans differing in glucosyltransferase (GTase) and fructosyltransferase (FTase) activities were examined. These strains had been made resistant to streptomycin. FTase activity of an S.

Flow- and pressure-adapted portal arterialization in dogs.

The effects of portal arterialization after portacaval shunt were studied in dogs. Flow- and pressure-adapted portal arterialization was performed by mounting a Teflon cuff on an autogenous vein bypass graft between the hepatic stump of the portal vein and the right renal artery. Immediately following operation, the total hepatic blood flow and intrahepatic portal venous pressure were within normal range.

[Evaluation of combined radiotherapy of gastric carcinoma].

Combined radiotherapy with chemotherapy was performed on 82 patients with advanced gastric carcinoma (T2-T4: M0). A small target volume, including the primary lesion and regional lymph nodes (N1), was irradiated with the dose of 60 Gy. The survival rate was 21% at two years and 6% at five.

Isoferritins from tumorous and non-tumorous human liver.

Human ferritins purified from normal liver (LF) and from hepatocellular carcinoma (HF) showed different biochemical and immunological characteristics. Subunit preparations from the isoferritins showed differences in isoelectric focusing patterns, although both HF and LF were found to be composed of H and L subunits with apparently the same molecular weights. It is postulated that heterogeneities between the two isoferritins are not merely due to different proportions of H and L subunits, but also result from the existence of distinct species of the subunits bearing different surface electric charges.

Radiation therapy of carcinoma in the rectum and sigmoid colon.

Radiation therapy combined with some anti-cancer drugs was performed in a total of 79 patients with carcinoma of the rectum and sigmoid colon, consisting of 41 primary, 25 recurrent and 13 postoperatively irradiated ones. Relief of subjective symptoms was observed in 50% or more of primary and recurrent patients. Radiation response of tumor was marked in 23 (56%) of primary ones.

[Treatment of operable breast cancer by tumorectomy and irradiation].

Six patients with operable breast cancer were treated by tumorectomy followed by radiotherapy at Miyagi Seijinbyo Center between 1972 and 1982. All patients are alive but local recurrence at the irradiated breast and lung metastasis occurred in one patient each. Acute skin reaction was evident in two patients and slight fibrosis of the irradiated breast was observed in another patient.

[Radiotherapy for esophageal carcinoma with broncho-esophageal fistula].

We studied treatment methods after fistula formation in 14 esophageal carcinoma patients with broncho-esophageal fistulae. Among 12 patients, 8 had no treatment, one was operated and 3 underwent radiotherapy using conventional fractionation. In only one case was there temporary closure of the fistula; none of the patients survived for more than 1 year.

[Results of combined therapy of irradiation and bleomycin suppository for advanced uterine cervical cancer].

Efficacy, survival rats and adverse effects of the combined therapy of irradiation with intravaginal bleomycin suppositories were analyzed and discussed in 49 patients with uterine cervical cancer. The results were as follows: Histological examination of biopsy specimens from the uterine cervix taken just after the completion of this treatment showed favorable control over the primary lesions. However, the efficacy of the bleomycin suppositories was too mild to form a basis for treatment without intracavitary irradiation.