[Ultrastructural changes in the jejunal mucosa of the dog after dissection of the cranial mesenteric plexus].

Ultrastructural change was investigated in the small intestinal mucosa of dogs after experimental dissection of the cranial mesenteric plexus and/or obstruction of the lymphatics. In group with a combination of dissection of the plexus and obstruction of the lymphatics (Group A): Marked mucosal edema and dilatation of intercellular spaces of epithelial cell were observed after the 6th postoperative hours (POH), and numerous pinocytotic vesicles appeared after 24th POH. After the 5th postoperative days, rough glycocalyx and irregular microvilli were recognized in epithelial cells.

A case of ovarian granulosa cell tumor with invasive adenocarcinoma of the endometrium. A case report and review of the Japanese literature.

A 47-year-old woman was admitted complaining of irregular uterine bleeding. On surgery, an ovarian tumor was found together with a broad-based polypoid endometrial mass. These lesions were histologically diagnosed as granulosa cell tumor with invasive adenocarcinoma of the endometrium.

Identification of secretory immunoglobulin A in human sweat and sweat glands.

Secretory immunoglobulin A (sIgA) plays an important role in local immune defense mechanisms. Although skin is always exposed to external antigens, the role of local immune defenses involving sIgA in the skin has not been adequately studied. In order to evaluate the presence of sIgA in sweat, we have measured the concentration of sIgA in human sweat by enzyme immunoassay and have localized the components of sIgA in the sweat glands of human axillary skin.

Distribution of atrial natriuretic polypeptide (ANP)-containing cells in the rat heart and pulmonary vein. Immunohistochemical study and radioimmunoassay.

The distribution of atrial natriuretic polypeptide (ANP) was immunohistochemically surveyed in the rat heart and lung using an antiserum raised against alpha-human ANP. The ANP-immunoreactive cells were seen to be distributed in the atrial walls and proximal portions of the pulmonary vein and venae cavae, but were absent from the aorta, pulmonary arteries, trachea, bronchus, and alveolar cells. The immunoreactive cells were present in a narrow region just beneath the endothelium of the pulmonary vein and vena cavae, and, ultrastructurally and immunocytochemically, were seen to be striated muscle cells with ANP-containing specific granules similar to those seen in atrial cardiocytes.

Interdigitating cell sarcoma. A morphologic, immunohistologic, and enzyme-histochemical study.

A 58-year-old man presented with an unusual sarcoma of the cervical lymph node. The tumor also involved the mesenteric lymph node and jejunum. Tumor cells possessed intracytoplasmic S100 protein, Leu-3a (T4), and HLA-DR antigens.

The role of leukotoxin (9,10-epoxy-12-octadecenoate) in the genesis of coagulation abnormalities.

This study was designed to clarify whether or not leukotoxin (9, 10-epoxy-12-octadecenoate), which is biosynthesized by neutrophils, might be involved in the genesis of coagulating abnormalities. Twelve dogs were divided into 2 groups. In the test group (n = 6), 100 mumol/kg of leukotoxin was injected intravenously, and in the control group (n = 6), 100 mumol/kg of linoleate was injected.

[A case of ovarian granulosa cell tumor with invasive adenocarcinoma of the endometrium].

A case of an ovarian granulosa cell tumor with an invasive adenocarcinoma of the endometrium, and a review of the relevant Japanese literature is reported. A 47-year-old woman was admitted to Ichinomiya Citizens' Hospital complaining of irregular uterine bleeding. On operating, an ovarian tumor was found with a broad-based polypoid mass extending into the endometrium.

Neutrophil-derived epoxide, 9,10-epoxy-12-octadecenoate, induces pulmonary edema.

We have observed that neutrophils biosynthesize linoleate epoxide, 9,10-epoxy-12-octadecenoate, and have named it leukotoxin because of its cytotoxic effect. In this experiment, the effect of leukotoxin on the lung was investigated. Acute effect of leukotoxin: Using Wistar rats, leukotoxin (100 mumol/kg) was injected intravenously for the leukotoxin group, and linoleate (100 mumol/kg) for the linoleate group.

Developmental expression of C3 receptor on murine epidermal Langerhans cells during ontogeny.

The developmental expression of C3 receptor, an important surface marker of murine epidermal Langerhans cells (LCs), was quantitatively studied using an immunohistochemical technique on epidermal sheets and then compared with developmental expression of Ia antigen and membrane ATPase. Anti-Mac-1 monoclonal antibody associated with CR3 was used for detecting C3 receptor and proved positive for LCs by immunoelectron microscopy. Mac-1 positive (Mac-1+) cells showed quite a different distribution from those of ATPase+ and Ia+ cells.

Presence of atrial natriuretic polypeptide in the pulmonary vein and vena cava.

Striated muscle cells and storage granules observed in the atria were found in main branches of the pulmonary veins and superior and inferior venae cavae of the rat, pig, and ox. The presence of atrial natriuretic polypeptide (ANP) in these veins was examined by reverse-phase high-performance liquid chromatography coupled with a radioimmunoassay for ANP. The veins contained 0.

Reorganization of thymic microenvironments during development and lymphomagenesis.

Modulation of thymic microenvironments during ontogeny and lymphomagenesis in mice was studied with two rat monoclonal antibodies (moAb) which recognized distinct subpopulations of thymic epithelial reticular cells (TER). In adult thymus, the TER subpopulation stained by moAb B6TS-1 was localized in the subcapsular zone, cortico-medullary junction, and medulla. In fetal thymus, it was initially distributed throughout the rudiment, but after day 16 of gestation, it was rapidly redistributed to the locations seen in adult thymus.

[A case of serous papillary cystadenocarcinoma of the ovary with hyperamylasemia].

A case of serous papillary cystadenocarcinoma of the ovaries with hyperamylasemia has been studied immunohistochemically, electron microscopically, and biochemically. Cellular localization of amylase in the tumor tissue was determined by biochemical analysis, using an indirect immunoperoxidase method. Electron microscopy revealed electron-dense granules located in the peripheral region of the cytoplasm of most tumor cells.

Thymic epithelial reticular cell subpopulations in mice defined by monoclonal antibodies.

Thymic epithelial reticular cells (TER) are heterogeneous cell populations. Of 14 rat monoclonal antibodies (moAbs) raised against established cell lines of mouse thymic stromal cells (TSC), two were found to recognize TER subpopulations that exhibited distinct intrathymic distributions. MoAb B6TS-1 (IgG2a) recognized the cell-surface determinant mB6TS-1 on TSC in the subcapsular zone, cortico-medullary junction, and medulla.

Enolase isozymes in renal tubules and renal cell carcinoma.

To elucidate the localization of enolase isozymes in renal tubules and renal cell carcinoma, an immunohistochemical study and quantitative analysis by employing the enzyme immunoassay were performed. The alpha-enolase was localized in almost all epithelial cells of renal tubules except for loops of Henle. The gamma-enolase was localized in macula densa cells and epithelial cells of loops of Henle and collecting ducts of the medulla, but not in those of proximal tubules.

Possible mechanisms of elevation of serum secretory immunoglobulin A in liver diseases.

In order to investigate possible mechanisms of elevation of serum secretory immunoglobulin A (sIgA) in liver diseases, human liver specimens were applied to immunohistochemical study of immunoglobulin A, secretory component, and J chain, which are components of sIgA. In the cases of chronic hepatitis with high serum sIgA levels, these antigens were present in dilated bile canaliculi of hepatocytes and they were continuously stained on the lateral plasma membrane of hepatocytes from the bile canaliculus to the space of Disse over the junctional complexes. Furthermore, in liver cirrhosis and extrahepatic cholestasis, they were also detected in intraportal bile ductules and intercellular spaces of degenerated cholangiocytes.

Observations on the fine structure of nodal, Purkinje and working myocardial cells isolated from rabbit hearts.

Sinoatrial and atrioventricular nodal cells as well as Purkinje and working myocardial cells isolated enzymatically from rabbit hearts were examined by transmission electron microscopy. The cardiac cell fine structure remained well organized in the storage solution after isolation. In normal Tyrode solution, the nodal cells changed from spindle shaped to spherical, whereas the shapes of Purkinje and working myocardial cells remained unchanged.

The transverse tubular system of rat myocardium: its morphology and morphometry in the developing and adult animal.

The three dimensional arrangements of the T system in the developing and adult animal were investigated by means of high voltage electron microscope stereoscopy using Golgi treated materials. The rat myocardial T system was composed of three major group elements: the transverse tubules, longitudinal tubules and flattened cisternae, which were classified according to their orientation and to their morphological features. It was found, as the growth of the rats proceeded, that the longitudinal tubules increased in number and that the transverse tubules were arranged more regularly and densely at the level of the z band.

Microbial adjuvant and autoimmunity. IV. The induction of thyroid lesions in syngeneic X-irradiated mice by the transfer of spleen cells from mice immunized with thyroid extract and Klebsiella O3 lipopolysaccharide.

The role of humoral and cellular immune responses in the initiation and maintenance of autoimmune thyroiditis was investigated in mice immunized with syngeneic thyroid extract and Klebsiella O3 lipopolysaccharide (KO3 LPS) as an adjuvant. The transfer of spleen cells from hyperimmunized mice to 400R-irradiated syngeneic mice produced definite lesions in the thyroid glands, whereas the transfer of immune sera failed to do so. No lesions were induced in normal intact mice by the same transfer of sera and spleen cells from hyperimmunized mice.

Hepatic vascular endothelial cells heterogenously express surface antigens associated with monocytes, macrophages and T lymphocytes.

During studies of the antigenic and functional properties of hepatic sinusoidal lining cells in situ, we found that only the sinusoidal endothelial cells share antigens with a peripheral blood macrophage subset capable of presenting soluble antigens and triggering autologous mixed lymphocyte reactions. They were HLA-DR+, OKM1-, OKM5+. Vascular endothelial cells in the portal areas and central veins were HLA-DR+, OKM1- and OKM5-.

Cellular and humoral dynamics in the periarterial lymphatic sheaths of rat spleens.

Humoral and cellular dynamics in the periarterial lymphatic sheath (PALS) of the splenic white pulp were investigated in regards to the lymph flow and the architecture of the deep lymphatics in rat spleens. The effects of the splenic venous pressure on the lymph flow were also examined. We found a remarkable depletion of lymphocytes in the PALS and strong lymph flow to the deep lymphatics from the red pulp when the spleen was perfused via the splenic artery at a high speed with a high venous pressure.