Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood.

We have developed a single-tube multiplex real-time PCR method for the detection of the eight most common Candida species causing septicemia: Candida albicans, C. dubliniensis, C. famata, C.

Identification of 43 Streptococcus species by pyrosequencing analysis of the rnpB gene.

Pyrosequencing technology was evaluated for identification of species within the Streptococcus genus. Two variable regions in the rnpB gene, which encodes the RNA subunit of endonuclease P, were sequenced in two reactions. Of 43 species, all could be identified to the species level except strains of the species pairs Streptococcus anginosus/S.