Dysregulated genes in HIGK-treated and their possible association with HNSCC.

The present study aims to identify the differentially expressed genes in HIGK treated with and their possible role in establishing head and neck squamous cell carcinoma. The study design follows a computational approach wherein multiple databases and tools are used to derive the possible association between exposure and the development of HNSCC. The GEOmnibus dataset GSE6927 provided data on the differentially expressed genes in the HIGK treated with .

Differential gene expression profile in treated human gingival keratinocytes and their role in the development of HNSCC.

Background: Periodontitis is considered to be one of the major risk factors associated with cancers of the oral cavity. Periodontogenic pathogens such as and are the important pathogens associated with periodontitis. Chronic exposure to bacterial components induces changes in the nearby cells.

Anticandidal effect of cinnamic acid characterized from Cinnamomum cassia bark against the fluconazole resistant strains of Candida.

Candida spp., causes invasive fungal infections, especially in immune-compromised patients and the propensity of antifungal resistance against azole-based drugs need to be addressed. This study is thus aimed to characterize the anticandidal effect of the cinnamic acid extracted from the barks of Cinnamomum cassia.

Analysis of differentially expressed genes in dysplastic oral keratinocyte cell line and their role in the development of HNSCC.

Dysplasia is the presence of abnormal types of cells in a tissue precipitated by over or diminished expression of certain genes. These cells act as a precursor to cancer. Dysplastic oral keratinocyte (DOK) cell lines have an aneuploid complex karyotype.

Alteration of SERPINH1 is associated with elevated expression in head and neck squamous cell carcinomas and its clinicopathological significance.

Background/purpose: Head and neck squamous cell carcinoma (HNSCC) is the sixth leading cause of cancer-related death worldwide and contributes significantly to the burden of disease in South Asia, partially due to the lack of effective screening strategies. Identifying essential biomarkers is crucial for improved prognosis and treatment. This study investigates the potential of SERPINH1 as a prognostic marker in HNSCC, highlighting its significance amidst the molecular complexity.

Deciphering the Genetic Alteration in the ZEB2 Gene Network and Their Possible Association With Head and Neck Squamous Cell Carcinoma (HNSCC).

Background Head and neck squamous cell carcinoma (HNSCC) is an abnormal growth of cells that leads to tumor formation in the head and neck region. Several genes and genetic networks are involved in the process of carcinogenesis. Aim The aim of the present study is to unravel the prognostic marker from a pool of interacting networks governed by the ZEB2gene Materials and methods Computational analysis was employed to identify the protein network interactions, genetic alterations, gene expression, and the survival analysis of the ZEB2 dysregulated network in the head and neck cancer dataset (HNSCC) from the Cancer Genome Atlas (TCGA), Firehose Legacy.

Anti-quorum sensing activity of against PAO1.

Quorum sensing (QS) is one of the key virulence factors in and causes recalcitrant infections. Multi-drug resistance and biofilm formation seem to be regulated by cell-to-cell communication system through QS. Thus this study is aimed to assess the efficacy of ethanolic leaf extract of in acting against the QS-regulated virulence traits.

The Implementation of HIV Self-Testing in Resource-Limited Settings Where the HIV Disease Burden is High.

In resource-limited settings, there is growing evidence that HIV testing is lacking among high-risk key populations such as men having sex with men, injection drug users, and transgenders largely due to stigma, discrimination, and lack of confidentiality. Findings from recent studies among high-risk key populations and the general population from various regions including resource-limited settings support the need for wider accessibility of HIV self-testing (HIV-ST) to reach those who may not otherwise have access to testing. Therefore, HIV-ST has untapped potential as a strategy to improve access to HIV testing and to increase testing frequency among key high-risk populations and their partners.

Moonlighting proteins [ML proteins]: The pandora's box of insidious oro-dental diseases.

Oral pathogens survive in the harsh niche of the oral microbiome on account of a plethora of moonlighting [ML] proteins that can multitask in the oro-mucosal layers. ML proteins are considered as the complex protein hyperspace expressed in many oral bacterial pathogens and encompass many hypothetical and experimentally evidenced proteins that can efficiently assist in the initiation and progression of various oro-dental infections. With the propensity of multi-drug resistance and biofilm formation, unravelling the mysterious functions associated with the oral ML proteins could be essential in targeting the vital oral bacteria and their associated infections.

Genistein binding protein targets in dental pathogens.

Oral pathogens have created a menace in recent years due to biofilm formation and antimicrobial drug resistance. The current treatment strategy works well with antibiotics. However, constant use of antibiotics creates a selective pressure, which increases adaptability of the pathogens.

Protein targets in red complex pathogens for catechin.

The development of antimicrobial drug resistance has encouraged scientists to develop alternate methods to combat infectious pathogens associated with dental diseases. Therefore, it is of interest to predict interactions for catechin (a plant derived compound) with protein targets in the red complex pathogens using computer aided network tools. However, in vitro and in vivo studies are warranted to confirm the antimicrobial effect of catechin (gallocatechin, epicatechin, epigallactocatechin (EGC) and gallolyl catechins) on the dental pathogens.

Virulence of Acinetobacter baumannii in proteins moonlighting.

The diverse function of the moonlighting proteins in Acinetobacter baumannii is highly associated with its virulence that had spurred renewed attention in recent years. The existing and newly formed hypothetical moonlighting proteins, evolve without jeopardizing the structural constraints of their original roles. It is yet uncertain and undefined to lucidly describe the functions of the moonlighting proteins in A.

Characterization of biofilm producing methicillin resistant coagulase negative Staphylococci from India.

Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) cause infectious diseases due to their potential to form biofilm and further colonization in hospital materials. This study evaluated the antibiotic susceptible phenotypes, biofilm-producing ability, and biofilm-associated genes (mecA, icaAD, bap, cna, and fnbA). Biofilm formation was detected through Congo red agar (CRA) method and MTP method.

Protean role of epigenetic mechanisms and their impact in regulating the Tregs in TME.

Constitutive expression of Foxp3+ Tregs in the tumor microenvironment (TME) specifically renders immune suppression in the tumor tissues. Being highly stable and self-tolerant, Tregs may be influenced by various epigenetic-associated mechanisms while exhibiting their functions. DNA methylation, histone acetylation, epigenetic silencing, alteration in chromatin networks, etc.

Genetic alterations in Wnt family of genes and their putative association with head and neck squamous cell carcinoma.

Head and neck squamous cell carcinoma (HNSCC) is the most frequent type of head and neck cancer that usually arises from the mucosal surfaces of several organs including nasal cavity, paranasal sinuses, oral cavity, tongue, pharynx, and larynx. The Wnt signaling pathway is a crucial mechanism for cellular maintenance and development. It regulates cell cycle progression, apoptosis, proliferation, migration, and differentiation.

Deciphering the genetic alterations in matrix metallo-proteinase gene family and its putative association with head and neck squamous cell carcinoma.

Matrix metallo-proteinases (MMPs) a group of zinc-dependent proteolytic enzymes which play a key role in tumorigenesis by degrading almost all extracellular matrix (ECM) components. MMPs are associated with tumour progression including invasion, angiogenesis, metastasis and poor prognosis. Genetic alterations such as single nucleotide variations and other gross chromosomal abnormalities have been found to drive the process of malignant transformation.

Delineating the Immuno-Dominant Antigenic Vaccine Peptides Against gacSSensor Kinase in : An Investigational Approach.

Objectives: To predict the novel vaccine peptide candidates against gacS protein involved with the citrate utilization in the two-component system of -associated virulence as an alternative strategy to combat the multi-drug resistant strains using an immuno-informatic approach.

Methods: The study is designed as an observational study design with the application of BepiPred, AlgPred, VaxiJen, AntigenPro, SolPro, Expasy ProtParam server, IEDB database, and MHC cluster analytical tools and servers to predict the immuno-dominant B-cell and T-cell epitopes from gacS FASTA sequences retrieved from UNIPROT database. Further peptide interactions with TLR-4 was assessed based on the number of hydrogen bonds.

Computational approach towards identification of pathogenic missense mutations in gene and their possible association with amelogenesis imperfecta.

Amelogenin gene encodes an enamel protein called amelogenin, which plays a vital role in tooth development. Any mutations in this gene or the associated pathway lead to developmental abnormalities of the tooth. The present study aims to analyze functional missense mutations in genes and derive an association with amelogenesis imperfecta.

A computational study targeting the mutated L321F of ERG11 gene in C. albicans, associated with fluconazole resistance with bioactive compounds from Acacianilotica.

Background: Emergence of fluconazole resistance mediated by L321F mutation in the ERG11 gene poses a serious impediment in the candidal treatment process. This leads to the search of novel target proteins to develop newer drugs against fluconazole resistant C. albicans.

An in silico approach towards identification of virulence factors in red complex pathogens targeted by reserpine.

Oral cavity hosts an exhaustive collection of microorganisms which are known to be associated with disease such as dental caries, periodontal and deep-seated infections. Elimination of these pathogens from the site of infection remains a perplexing task, which demands the use of antibiotics. The emergence of drug resistant forms has spurred interest into identifying novel therapeutic targets against these pathogens.

An insight into the emergence of as an oro-dental pathogen and its drug resistance gene profile - An in silico approach.

Background: , a potential nosocomial pathogen has stealthily gained entry into the oral cavity. Their association with other pathogens like in chronic and aggressive periodontitis cases is well documented. The magnitude of problem caused by could be attributed to resistance genes acquired by the organism.

In silico analysis of virulence genes in an emerging dental pathogen A. baumannii and related species.

Objectives: Acinetobacter baumannii is an opportunistic pathogen which has recently been categorized as a high risk pathogen by World Health Organisation (WHO). The microbe has stealthily entered the oral cavity and has established itself as a potential pathogen by acquiring drug resistance and expression of several virulence genes. Surveillance on the type of virulence factors harboured by the organism will enable us to comprehend the mechanism of pathogenesis.

Antimicrobial Effect of Leaves of and on Caries Causing Bacteria: An In vitro Study.

Introduction: and are which are indigeneous to India. commonly called 'manathakkali Keerai' in Tamil, forms an indispensable part of South Indian diet. (keezhanelli in Tamil) is a widely used medicinal plant, the leaves of which have been used extensively in Ayurveda and native medicine to cure various liver ailments.