[Reactive-dystrophic processes in salivary glands (sialoadenoses) running on the background of metabolic syndrome].

Basing upon comprehensive checkup of 82 patients with metabolic syndrome authors made diagnostics of different forms of sialoadenosis in them. The disease proceeded on the background of abdominal adiposity, lipid metabolism derangements, glucose toleration disturbances and diabetes mellitus of the 2nd type with reduction of tissue sensitivity to insulin (insulin resistivity). Results of the fulfilled patient treatment showed that it was necessary to include in the complex of therapeutic measures metformine (glukofagee) producing positive effect upon carbohydrate metabolism and also to normalize the work of glands increasing their secretory activity.

[Effect of a postsynaptic alpha2-adrenoreceptor antagonist beditin on the rat resistance to seizure and Ca(+)-level in the brain tissue].

In rats with a hemodynamic disorder caused by acute myocardial ischemia, preliminary administration of beditin in a dose of 25 mg/kg fully prevents a manifold increase in the level of 45Ca2+ in the cytosol of brain cells and leads to enhanced trapping and accumulation of labeled calcium in endoplasmic reticulum. With respect to calcium binding in endoplasmic reticulum, the action of beditin substantially differs from that of verapamil.

[Effect of beditine on the cerebral circulation and various metabolic parameters].

Beditin, or 2-(2-amino-4-thiazolyl)-1,4-benzodioxane hydrochloride, is a new benzodioxane derivative possessing antihypoxant and alpha 2-adrenoreceptor blocker properties. In contrast to idazoxan, a well-known alpha 2-adrenoreceptor blocker, beditin (3 mg/kg) significantly increases the circulation rate and decreases the blood vessel resistance in various brain structures of rats and cats without considerably changing their systemic hemodynamic indices. Upon a one-side ligation of the common carotid artery in rats, beditin prevented a sharp increase in the lactate dehydrogenase activity and a growth in the content of free ammonia in the brain, which are characteristic of the ischemic states.

[The effect of verapamil on changes in the Ca2+ content of the intracellular structures of organs during acute myocardial ischemia].

In experiments on rats with acute myocardial infarction induced by occlusion of the left coronary artery in conscious animals the protective effect of verapamil on the heart, brain, liver, and kidneys in this disease was studied. The effectiveness of the drug was judged by the changes in the 45Ca2+ content in the intracellular structures (cytosol, mitochondria, endoplasmic reticulum) of the organs under study. A single injection of 200 micrograms/kg verapamil in acute myocardial infarction reduces considerably the content of 45Ca2+ in the intracellular structures of these organs and thus prevents the development of pathological processes.

[Theory of cooperative transition of DNA complexes with multimodal ligands].

The theory of the cooperative transition of DNA.ligand complexes have been developed, in which a model was implied where the multimodal ligands simultaneously interacted with DNA. Obtained formula express the dependence of the experimentally estimated values of the changes of transition point and width of transition on the concentration of the ligands.

[Interaction of DNA with divalent metal ions].

The interaction between the native DNA macromolecules and Ca2+, Mn2+, Cu2+ ions in solutions of low ionic strength (10(-3) M Na+) is studied using the methods of differential UV spectroscopy and CD spectroscopy. It is shown that the transition metal ions Mn2+ exercise binding to the nitrogen bases of DNA at concentrations approximately 5 x 10(-6) M and form chelates with guanine of N7-Me(2+)-O6 type. Only at high concentrations in solution (5 x 10(-3) M) do Ca2+ ions interact with the nitrogen bases of native DNA.

[The clinical and prognostic information value of painless myocardial ischemia in the early postinfarct period].

A study was made of painless myocardial ischemia in the early postinfarction period (day 14) in order to define its influence on the clinical course and immediate prognosis. 25 patients aged 32 to 60 years with acute primary myocardial infarction were examined. 17 patients had large-focal and 8 small-focal infarction.

[The participation of the hypophyseal-adrenal system in regulating alpha 2-adrenoreceptor reactivity. The effects of extreme exposures and alpha 2-adrenoblockaders].

The simulation of the hypo- and hyperactivity of the hypophysis-adrenocortical system induced considerable changes in the reactivity of alpha 2-adrenoceptors, as well as in the resistance of the organism to stressful influences. The findings support the assumption that the blockade of alpha 2-adrenoceptors is one of the key features in the adaptive mechanism of the glycocorticoid hormones action.

[Selective effect of ionization of nitrogen bases on the thermostability of AT- and GC-pairs of DNA].

The ionization specific influence of nitrogen bases on thermostability of AT- and GC-pairs of DNA has been investigated by the method of DNAs melting temperature analysis. It has been shown that the change of temperature interval of DNA helix-coil transition when changing pH environment is due to specific ionization of AT- and GC-pairs of nitrogen bases.

[The effect of long-range interactions between adsorbed ligands on the DNA helix-coil transition].

The influence of different types of long-range interaction of ligands adsorbed on DNA on the helix-coil transition was theoretically considered. The contact interaction was shown to differ significantly from the long-rang one. It was shown also that even weak dependence of a long-range potential on a degree of helicity resulted in the strong changes of a DNA melting curve.

[The diagnosis and treatment of cystic diseases of the kidneys by using transcutaneous puncture methods under ultrasonic monitoring].

Presented in the paper are the methods of ultrasonic diagnosis and percutaneous paracentetic treatment of cystic kidneys under ultrasonic monitoring. Paracenteses were performed in 172 patients: 59 with polycystic and 113 with cystic kidneys. If the cysts located in the middle or lower segments of posterior, lateral or inferior drainage that was pushed through the guide into the cyst lumen.

[DNA helix-coil transition in alkaline medium].

Dna helix-coil transition in th alkaline medium was considered theoretically and experimentally. On the basis of the theory and experimental comparison the DNA double-stranded form deprotonation was revealed.

[Effect of aflatoxin B1 on DNA enzymatic methylation and secondary structures of the rat liver].

Methylation and secondary structure of DNA in rat liver versus time of aflatoxin B1 (AfB1) treatment were studied. A 50% drop in 5-methylcytosine level in liver DNA as compared with control was observed long before tumor development. Also, certain changes in DNA secondary structure were seen.

[Selective effect of ligands on AT- and GC-pairs of DNA bases].

A method is proposed for investigating the specific influence of ligands on DNA AT- and GC-pairs based on the comparison of the width of transition temperature interval of DNA with a strongly pronounced block-structure and dependence of the control DNA melting temperature on their GC-content. Such a comparison allows to define separately the contribution of nonspecific effects causing a change of the width of transition temperature interval. The method is used for studying the specific influence of beta-alanine and nu-aminobutyric acid on AT- and GC-pairs.

[DNA conformation and thermostability of aqueous solutions of beta-alanine and gamma-aminobutyric acid].

It has been shown that at low concentrations of rare amino acids (from 10(-3) M to 10(-1) M of the substance) stechiometric complexes amino acid -- DNA are formed, which bring about partial substitution of counterions screening phosphate groups and to a change of spatial structure of DNA water molecules. The DNA-solvent molecular interactions are changed, accompanied by an abrupt decrease of helix-coil enthalpy transition which leads to the unwinding of DNA double helix. In the region of amino acid high concentrations (greater than 1-1,5 M) a rise of thermostability and winding of DNA double helix is observed.

[DNA conformation and thermostability in urea aqueous solutions].

It is suggested from the character of the change of circular dichroism spectra that in the presence of urea winding of DNA double helix takes place within the bounds of B-family of forms. It is shown that the realized conformation of DNA differs from the experimentally known forms of DNA belonging to B-family. Urea destabilizes the DNA molecules without connection with helical and melt pairs of DNA nitrous bases.

[Modification of DNA-specific methylation in tissues of healthy and tumor bearing (sarcoma 45) rats under the effect of an active alkylating cancerolytic agent].

The content of 5-methyl cytosine (5-MC) in the DNA preparations from organs and tissues of healthy and tumour-bearing (sarcoma 45) rats and the effect of an alkylating cancerolytic agent on DNA methylation in vivo were studied. The 5-MC content in the DNA preparations from liver, spleen and testicles of tumour-bearing rats and tumour cells was increased, the maximal increase being observed in liver and spleen DNAs (1,71 and 1,70 mol.%, respectively) and in sarcoma DNA (1,93 mol.